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anti-Human DAAM1 antibody for Western Blotting

Recommended DAAM1 Antibody (supplied by: Log in to see )

Antigen
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
  • daam-1
  • xdaam1
  • DAAM1
  • DKFZp468D0524
  • 1700066F09Rik
  • 2310028E21Rik
  • AI503486
  • E130308H01
  • mKIAA0666
  • daam1
  • dishevelled associated activator of morphogenesis 1
  • dishevelled associated activator of morphogenesis 1a
  • daam1
  • DAAM1
  • Daam1
  • daam1a
Epitope
AA 341-370, N-Term
27
11
7
3
3
3
3
2
2
2
2
1
1
1
Reactivity
Hamster, Human
76
16
16
8
5
4
4
1
1
1
1
1
1
1
1
Host
Rabbit
70
6
Clonality
Polyclonal
Conjugate
This DAAM1 antibody is un-conjugated
5
5
5
5
5
5
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
71
49
46
8
8
6
4
2
2
1
1
Options
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Catalog No. ABIN651631
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN389179 IHC (p) WB Rabbit Ig AA 45-74, N-Term Log in to see Polyclonal 1
3.0726016 ABIN2786070 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0726016 ABIN389180 IHC (p) WB Rabbit Ig AA 1034-1063, C-Term Log in to see Polyclonal 1
3.0726016 ABIN564982 ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 5D3 2
3.0726016 ABIN2459853 ELISA WB Rabbit Log in to see Polyclonal
3.0726016 ABIN3222770 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN630809 WB Rabbit Middle Region Log in to see Polyclonal
3.0726016 ABIN4890151 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN4304202 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN524909 PLA ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 4H3
3.0726016 ABIN2434528 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN2432917 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN2473223 WB Mouse IgG2a AA 1-111 Log in to see 4H3
1 ABIN357856 EIA IHC (p) WB Rabbit Ig C-Term, N-Term Log in to see Polyclonal 3
1 ABIN357855 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
1 ABIN463923 WB Rabbit IgG AA 396-445 Log in to see Polyclonal
1 ABIN960124 WB Rabbit AA 1-50 Log in to see Polyclonal
1 ABIN1962490 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1968970 IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1964867 IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-DAAM1 antibody for Western Blotting

Similar anti-DAAM1 Antibodies

Application / Reactivity Human Hamster
ELISA 49 Antibodies 7 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 9 Antibodies 9 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 46 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 72 Antibodies 9 Antibodies

General

Antigen Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
Epitope AA 341-370, N-Term
(27), (11), (7), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Hamster, Human
(76), (16), (16), (8), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(70), (6)
Clonality (Clone)
Polyclonal   ( )
Conjugate This DAAM1 antibody is un-conjugated
(5), (5), (5), (5), (5), (5)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(71), (49), (46), (8), (8), (6), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-DAAM1 Antibody

Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN651631) Images
Specificity This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-378 amino acids from the N-terminal region of human DAAM1-T361.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 AA from the N-terminal region of human DAAM1-T361.
Clone RB15348
Isotype Ig

Target Details DAAM1

Product Details anti-DAAM1 Antibody Application Details Handling References for anti-DAAM1 Antibody (ABIN651631) Images back to top
Antigen
Alternative Name DAAM1 (DAAM1 Antibody Abstract)
Background Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
Synonyms: DAAM1,KIAA0666,Disheveled-associated activator of morphogenesis 1
Molecular Weight 123473 DA
Gene ID 23002
NCBI Accession NP_055807
UniProt Q9Y4D1
Research Area Cell Structure
Pathways WNT Signaling

Application Details

Product Details anti-DAAM1 Antibody Target Details DAAM1 Handling References for anti-DAAM1 Antibody (ABIN651631) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details References for anti-DAAM1 Antibody (ABIN651631) Images back to top
Format Liquid
Concentration 0.26 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-DAAM1 Antibody (ABIN651631)

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling Images back to top
Background publications

Higashi, Ikeda, Shirakawa, Kondo, Kawato, Horiguchi, Okuda, Okawa, Fukai, Nureki, Kita, Horiuchi: "Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8746-55, 2008

Liu, Sato, Khadka, Bharti, Diaz, Runnels, Habas: "Mechanism of activation of the Formin protein Daam1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 210-5, 2008

Images

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN651631) back to top
Supplier Images
Western Blotting (WB) image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (AA 341-370) (ABIN651631) DAAM1-T361 Antibody (N-term) (ABIN651631) western blot analysis in WiDr cell line lys...
Western Blotting (WB) image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (AA 341-370) (ABIN651631) DAAM1-T361 Antibody (N-term) (ABIN651631) western blot analysis in CHO cell line lysa...
Immunohistochemistry (IHC) image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (AA 341-370) (ABIN651631) DAAM1-T361 Antibody (N-term) (ABIN651631) immunohistochemistry analysis in formalin f...