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anti-Human DAAM1 antibody for Western Blotting

Recommended DAAM1 Antibody (supplied by: Log in to see )

Antigen
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
  • 1700066F09Rik
  • 2310028E21Rik
  • AI503486
  • daam-1
  • daam1
  • DAAM1
  • DKFZp468D0524
  • E130308H01
  • mKIAA0666
  • xdaam1
Epitope
Middle Region
35
12
8
3
3
3
2
2
2
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
71
10
10
2
Host
Rabbit
65
6
Clonality
Polyclonal
Conjugate
This DAAM1 antibody is un-conjugated
5
5
5
5
5
5
Application
Western Blotting (WB)
64
50
43
9
8
4
2
1
1
1
Supplier
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Catalog No. ABIN2786070
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.123033054 ABIN651631 FACS IHC (p) WB Rabbit Ig AA 341-370, N-Term Log in to see Polyclonal 2
0.123033054 ABIN389179 IHC (p) WB Rabbit Ig AA 45-74, N-Term Log in to see Polyclonal 1
0.123033054 ABIN389180 IHC (p) WB Rabbit Ig AA 1034-1063, C-Term Log in to see Polyclonal 1
0.123033054 ABIN564982 ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 5D3 2
0.123033054 ABIN2459853 ELISA WB Rabbit Log in to see Polyclonal
0.123033054 ABIN630809 WB Rabbit Middle Region Log in to see Polyclonal
0.123033054 ABIN524909 PLA ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 4H3
0.123033054 ABIN2158477 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.123033054 ABIN2158475 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.123033054 ABIN2158476 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.123033054 ABIN2473223 WB Mouse IgG2a AA 1-111 Log in to see 4H3
0.123033054 ABIN2434528 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.123033054 ABIN2432917 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011806352 ABIN357856 EIA IHC (p) WB Rabbit Ig C-Term, N-Term Log in to see Polyclonal 3
0.0011806352 ABIN357855 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.0011806352 ABIN463923 WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011806352 ABIN1975882 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 348-378 Log in to see Polyclonal
0.0011806352 ABIN960124 WB Rabbit AA 1-50 Log in to see Polyclonal
0.0011806352 ABIN1964869 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 348-378 Log in to see Polyclonal
0.0011806352 ABIN1962492 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 348-378 Log in to see Polyclonal

Top referenced anti-DAAM1 antibody for Western Blotting

Similar anti-DAAM1 Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Dog (Canine) Mouse (Murine) Rat (Rattus) Zebrafish (Danio rerio)
Western Blotting (WB) 65 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 9 Antibodies 9 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunohistochemistry (IHC) 43 Antibodies 5 Antibodies 5 Antibodies

General

Antigen Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
Epitope Middle Region
(35), (12), (8), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(71), (10), (10), (2)
Host Rabbit
(65), (6)
Clonality
Polyclonal
Conjugate This DAAM1 antibody is un-conjugated
(5), (5), (5), (5), (5), (5)
Application Western Blotting (WB)
(64), (50), (43), (9), (8), (4), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DAAM1 Antibody

Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN2786070) Images
Blocking Peptide Blocking peptide for this product available: ABIN975574
Sequence GNTVQYWLLL DRIIQQIVIQ NDKGQDPDST PLENFNIKNV VRMLVNENEV
Predicted Reactivity Bovine : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Dog : 90 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against DAAM1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human DAAM1

Target Details DAAM1

Product Details anti-DAAM1 Antibody Application Details Handling References for anti-DAAM1 Antibody (ABIN2786070) Images back to top
Antigen
Alternative Name DAAM1 (DAAM1 Antibody Abstract)
Background Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein.Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
Molecular Weight 123 kDa
Gene ID 23002
NCBI Accession NP_055807, NM_014992
UniProt Q9Y4D1
Pathways WNT Signaling

Application Details

Product Details anti-DAAM1 Antibody Target Details DAAM1 Handling References for anti-DAAM1 Antibody (ABIN2786070) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1078 AA

Restrictions For Research Use only

Handling

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details References for anti-DAAM1 Antibody (ABIN2786070) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DAAM1 Antibody (ABIN2786070)

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling Images back to top
Background publications

Higashi, Ikeda, Shirakawa, Kondo, Kawato, Horiguchi, Okuda, Okawa, Fukai, Nureki, Kita, Horiuchi: "Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8746-55, 2008

Images

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling References for anti-DAAM1 Antibody (ABIN2786070) back to top
Supplier Images
Western Blotting (WB) image for anti-DAAM1 antibody (Dishevelled Associated Activator of Morphogenesis 1) (Middle Region) (ABIN2786070) WB Suggested Anti-DAAM1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: