anti-Human DAAM1 antibody for Western Blotting

Recommended DAAM1 Antibody (supplied by: Log in to see )

Antigen
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
  • daam-1
  • xdaam1
  • DAAM1
  • DKFZp468D0524
  • 1700066F09Rik
  • 2310028E21Rik
  • AI503486
  • E130308H01
  • mKIAA0666
  • daam1
  • dishevelled associated activator of morphogenesis 1
  • dishevelled associated activator of morphogenesis 1a
  • daam1
  • DAAM1
  • Daam1
  • daam1a
Epitope
Middle Region
29
12
8
4
3
3
3
3
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
77
15
15
9
4
4
3
1
1
1
1
1
1
Host
Rabbit
71
6
Clonality
Polyclonal
Conjugate
This DAAM1 antibody is un-conjugated
5
5
5
5
5
5
Application
Western Blotting (WB)
72
49
46
11
9
6
4
2
2
1
1
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Catalog No. ABIN2786070
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9709127 ABIN389179 IHC (p) WB Rabbit Ig AA 45-74, N-Term Log in to see Polyclonal 1
2.9709127 ABIN651631 FACS IHC (p) WB Rabbit Ig AA 341-370, N-Term Log in to see Polyclonal 2
2.9709127 ABIN389180 IHC (p) WB Rabbit Ig AA 1034-1063, C-Term Log in to see Polyclonal 1
2.9709127 ABIN564982 ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 5D3 2
2.9709127 ABIN3222770 WB Rabbit Log in to see Polyclonal
2.9709127 ABIN2459853 ELISA WB Rabbit Log in to see Polyclonal
2.9709127 ABIN2473223 WB Mouse IgG2a AA 1-111 Log in to see 4H3
2.9709127 ABIN630809 WB Rabbit Middle Region Log in to see Polyclonal
2.9709127 ABIN4890151 WB Rabbit Log in to see Polyclonal
2.9709127 ABIN4304202 WB Rabbit Log in to see Polyclonal
2.9709127 ABIN524909 PLA ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 4H3
2.9709127 ABIN2434528 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9709127 ABIN2432917 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN357856 EIA IHC (p) WB Rabbit Ig C-Term, N-Term Log in to see Polyclonal 3
1 ABIN357855 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
1 ABIN463923 WB Rabbit IgG AA 396-445 Log in to see Polyclonal
1 ABIN960124 WB Rabbit AA 1-50 Log in to see Polyclonal
1 ABIN5027643 WB Rabbit IgG AA 1-230 Log in to see Polyclonal
1 ABIN1971283 IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1968970 IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal

General

Antigen Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) Antibodies
Epitope Middle Region
(29), (12), (8), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(77), (15), (15), (9), (4), (4), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(71), (6)
Clonality
Polyclonal
Conjugate This DAAM1 antibody is un-conjugated
(5), (5), (5), (5), (5), (5)
Application Western Blotting (WB)
(72), (49), (46), (11), (9), (6), (4), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-DAAM1 Antibody

Target Details DAAM1 Application Details Handling References for anti-DAAM1 antibody (ABIN2786070) Images
Blocking Peptide Blocking peptide for this product available: ABIN975574
Sequence GNTVQYWLLL DRIIQQIVIQ NDKGQDPDST PLENFNIKNV VRMLVNENEV
Predicted Reactivity Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against DAAM1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human DAAM1

Target Details DAAM1

Product Details anti-DAAM1 Antibody Application Details Handling References for anti-DAAM1 antibody (ABIN2786070) Images back to top
Antigen
Alternative Name DAAM1 (DAAM1 Antibody Abstract)
Background Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein.Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
Alias Symbols: FLJ41657, KIAA0666
Protein Interaction Partner: UBC, PRPF40A, B3GALT4, AMBP, MAP3K7, DVL2, DVL1, RHOA,
Protein Size: 1078
Molecular Weight 123 kDa
Gene ID 23002
NCBI Accession NM_014992, NP_055807
UniProt Q9Y4D1
Pathways WNT Signaling

Application Details

Product Details anti-DAAM1 Antibody Target Details DAAM1 Handling References for anti-DAAM1 antibody (ABIN2786070) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1078 AA

Restrictions For Research Use only

Handling

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details References for anti-DAAM1 antibody (ABIN2786070) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DAAM1 antibody (ABIN2786070)

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling Images back to top
Background publications

Higashi, Ikeda, Shirakawa, Kondo, Kawato, Horiguchi, Okuda, Okawa, Fukai, Nureki, Kita, Horiuchi: "Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8746-55, 2008

Images

Product Details anti-DAAM1 Antibody Target Details DAAM1 Application Details Handling References for anti-DAAM1 antibody (ABIN2786070) back to top
Supplier Images
Western Blotting (WB) image for anti-Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) (Middle Region) antibody (ABIN2786070) WB Suggested Anti-DAAM1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: