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Human DKK1 ELISA Kit

Recommended DKK1 Product (supplied by: Log in to see )

Antigen
Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) ELISA Kits
  • AI414495
  • CT34
  • dickkopf-1
  • Dickkopf1
  • dkk
  • DKK-1
  • dkk-1
  • dkk1
  • dkk1-A
  • mdkk-1
  • mSgy
  • SGY
  • SGY-1
  • SGY1
  • SK
  • Soggy
  • Xdkk-1
  • xdkk1
Epitope
AA 32-266
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
22
19
10
4
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672821
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3094543 ABIN414760 Sandwich ELISA Serum, Plasma, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.3094543 ABIN1114624 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.3094543 ABIN1380028 Sandwich ELISA Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.3094543 ABIN2708193 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012307835 ABIN858695 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012307835 ABIN365085 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012307835 ABIN3032911 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 122.9-30000 pg/mL 122.9 pg/mL Log in to see
0.0012307835 ABIN510425 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1.0-25 ng/mL 1 ng/mL Log in to see
0.0012307835 ABIN3198421 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Log in to see
0.0012307835 ABIN624961 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.0012307835 ABIN454969 Serum, Plasma, Tissue Homogenate, Biological Fluids 78-5000 pg/mL 78 pg/mL Log in to see
0.0012307835 ABIN1028543 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012307835 ABIN2684053 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012307835 ABIN2042438 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012307835 ABIN2113668 Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.0012307835 ABIN2684054 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.0012307835 ABIN1129667 78-5000 pg/mL 78 pg/mL Log in to see
0.0012307835 ABIN2543842 Sandwich ELISA Serum, Plasma, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.0012307835 ABIN2543841 Serum, Plasma, Biological Fluids 18-1000 pg/mL 18 pg/mL Log in to see
0.0012307835 ABIN2947006 31.25-2000 pg/mL 31.25 pg/mL Log in to see

Top referenced DKK1 ELISA Kit for ELISA

  • Human DKK1 ELISA Kit for Sandwich ELISA - ABIN624961 (2 Publications): de Andrade, de Castro, Vicente, da Rosa, Nader, Pereira, Fröde: Evaluation of circulating levels of inflammatory and bone formation markers in axial spondyloarthritis. in International immunopharmacology 2014 (PubMed)

Similar DKK1 ELISA Kits

Application / Reactivity Human
ELISA 23 ELISA Kits

General

Antigen Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) ELISA Kits
Epitope AA 32-266
Reactivity Human
Kits with alternative reactivity to:
(22), (19), (10), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Supplier Log in to see

Product Details DKK1 ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of human DKK1 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Oligomeric forms of natural and recombinant human DKK1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human DKK1 antibod - 1
  • Lyophilized recombinant human DKK1 standar - 10ng/tubex2
  • Biotinylated anti- human DKK1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: T32-H266

Target details

Product Details DKK1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name DKK1 (DKK1 ELISA Kit Abstract)
Background Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Gene ID 22943
UniProt I1W660
Research Area Embryogenesis, Wnt
Pathways WNT Signaling

Application Details

Product Details DKK1 ELISA Kit Target details Handling Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK1 has been precoated onto 96-well plates. Standards(sf21,T32-H266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DKK1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 610, Standard deviation: 32.94, CV(%): 5.4
  • Sample 2: n=16, Mean(pg/ml): 1503, Standard deviation: 73.65, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 2715, Standard deviation: 103.2, CV(%): 3.8,
  • Sample 1: n=24, Mean(pg/ml): 723, Standard deviation: 59.3, CV(%): 8.2
  • Sample 2: n=24, Mean(pg/ml): 1862, Standard deviation: 126.6, CV(%): 6.8
  • Sample 3: n=24, Mean(pg/ml): 3035, Standard deviation: 169.96, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details DKK1 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details DKK1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for DKK1 ELISA Kit (Dickkopf Homolog 1 (Xenopus Laevis)) (ABIN1672821) Human DKK-1 PicoKine ELISA Kit standard curve