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Mouse (Murine) DKK1 ELISA Kit

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Antigen
Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) ELISA Kits
  • AI414495
  • CT34
  • dickkopf-1
  • Dickkopf1
  • dkk
  • DKK-1
  • dkk-1
  • dkk1
  • dkk1-A
  • mdkk-1
  • mSgy
  • SGY
  • SGY-1
  • SGY1
  • SK
  • Soggy
  • Xdkk-1
  • xdkk1
Epitope
AA 30-272
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
23
18
10
4
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
46.9-3000 pg/mL
Minimum Detection Limit
46.9 pg/mL
Application
ELISA
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Catalog No. ABIN1672840
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN1114626 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32748017 ABIN1569417 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.32748017 ABIN2708497 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012092679 ABIN424472 Sandwich ELISA Serum, Plasma, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
0.0012092679 ABIN813611 Sandwich ELISA Serum, Plasma, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012092679 ABIN858696 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN627758 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012092679 ABIN3198422 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 93.75-6000 pg/mL 93.75 pg/mL Log in to see
0.0012092679 ABIN625383 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see
0.0012092679 ABIN510805 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012092679 ABIN2042439 Sandwich ELISA 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012092679 ABIN2684055 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012092679 ABIN2762148 Serum, Plasma, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012092679 ABIN1129669 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2543843 Sandwich ELISA Serum, Plasma, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
0.0012092679 ABIN1129668 Log in to see
0.0012092679 ABIN2080440 Sandwich ELISA Log in to see
0.0012092679 ABIN2947001 62.5-4000 pg/mL 62.5 pg/mL Log in to see

Top referenced DKK1 ELISA Kit for ELISA

Similar DKK1 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 19 ELISA Kits

General

Antigen Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) ELISA Kits
Epitope AA 30-272
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(23), (18), (10), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 46.9-3000 pg/mL
Minimum Detection Limit 46.9 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details DKK1 ELISA Kit

Target details Application Details Handling References for DKK1 Kit (ABIN1672840) Images
Purpose For quantitative detection of mouse DKK1 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse DKK1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <15pg/mL
Components
  • 96-well plate precoated with anti- mouse DKK1 antibod - 1
  • Lyophilized recombinant mouse DKK1 standar - 10ng/tubex2
  • Biotinylated anti- mouse DKK1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: CHO
Immunogen sequence: S30-H272

Target details

Product Details DKK1 ELISA Kit Application Details Handling References for DKK1 Kit (ABIN1672840) Images back to top
Antigen
Alternative Name DKK1 (DKK1 ELISA Kit Abstract)
Background Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Gene ID 13380
UniProt O54908
Research Area Embryogenesis, Wnt
Pathways WNT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DKK1 has been precoated onto 96-well plates. Standards(CHO, S30-H272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DKK1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse DKK1 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 12.54, CV(%): 5.5
  • Sample 2: n=16, Mean(pg/ml): 845, Standard deviation: 56.6, CV(%): 6.7
  • Sample 3: n=16, Mean(pg/ml): 1474, Standard deviation: 91.39, CV(%): 6.2,
  • Sample 1: n=24, Mean(pg/ml): 259, Standard deviation: 17.1, CV(%): 6.6
  • Sample 2: n=24, Mean(pg/ml): 897, Standard deviation: 66.4, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 1532, Standard deviation: 105.7, CV(%): 6.9
Restrictions For Research Use only

Handling

Product Details DKK1 ELISA Kit Target details Application Details References for DKK1 Kit (ABIN1672840) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for DKK1 Kit (ABIN1672840)

Product Details DKK1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Roessler, Du, Glinka, Dutra, Niehrs, Muenke: "The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly." in: Cytogenetics and cell genetics, Vol. 89, Issue 3-4, pp. 220-4, 2000

Images

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Supplier Images
ELISA image for DKK1 ELISA Kit (Dickkopf Homolog 1 (Xenopus Laevis)) (ABIN1672840) Mouse DKK1 PicoKine ELISA Kit standard curve