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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||28 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies|
|Western Blotting (WB)||82 Antibodies|
|Antigen||Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) Antibodies|
|Epitope||AA 7-36, N-Term Alternatives|
|Conjugate||This DKK4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-DKK4 AntibodyTarget Details DKK4 Application Details Handling References for anti-DKK4 Antibody (ABIN357073) Images
|Specificity||This antibody detects DKK4 at N-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of Human DKK4|
Target Details DKK4Product Details anti-DKK4 Antibody Application Details Handling References for anti-DKK4 Antibody (ABIN357073) Images back to top
|Alternative Name||Dickkopf-4 (DKK4) (DKK4 Antibody Abstract)|
|Background||DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4|
|Molecular Weight||24875 Da|
|Gene ID||27121, 9606|
Application DetailsProduct Details anti-DKK4 Antibody Target Details DKK4 Handling References for anti-DKK4 Antibody (ABIN357073) Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DKK4 Antibody Target Details DKK4 Application Details References for anti-DKK4 Antibody (ABIN357073) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-DKK4 Antibody (ABIN357073)Product Details anti-DKK4 Antibody Target Details DKK4 Application Details Handling Images back to top
Yoshida, Satoh, Mitsuya et al.: "Assignment of the human dickkopf (Xenopus) homolog 4 (DKK4) to chromosome 8p11.2-->p11.1 by fluorescence in situ hybridization." in: Cytogenetics and cell genetics, Vol. 94, Issue 1-2, pp. 88-9, 2001 (PubMed).
Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).
ImagesProduct Details anti-DKK4 Antibody Target Details DKK4 Application Details Handling References for anti-DKK4 Antibody (ABIN357073) back to top