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anti-Human DKK4 antibody for Western Blotting

Recommended DKK4 Antibody (supplied by: Log in to see )

Antigen
Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) Antibodies
  • Dkk-4
  • DKK-4
  • RGD1563172
Epitope
C-Term
53
21
16
5
3
2
2
2
2
1
1
1
Reactivity
Human
92
19
6
2
1
1
1
1
Host
Goat
87
14
3
Clonality
Polyclonal
Conjugate
This DKK4 antibody is un-conjugated
8
8
7
7
7
7
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
89
60
29
10
3
2
2
Supplier
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Catalog No. ABIN184710
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12935057 ABIN951912 EIA WB Rabbit Ig C-Term, AA 169-197 Log in to see Polyclonal 5
0.12935057 ABIN388357 IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 3
0.12935057 ABIN249465 ELISA WB Goat IgG C-Term Log in to see Polyclonal 1
0.12935057 ABIN411467 WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN655596 WB Rabbit Ig AA 162-189, C-Term Log in to see Polyclonal
0.12935057 ABIN2775398 WB Rabbit C-Term Log in to see Polyclonal
0.12935057 ABIN388358 IHC (p) WB Rabbit Ig AA 188-217, C-Term Log in to see Polyclonal
0.12935057 ABIN2465606 ELISA WB Goat C-Term Log in to see Polyclonal
0.12935057 ABIN2160602 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12935057 ABIN2160601 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12935057 ABIN2160603 WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.12935057 ABIN949206 WB Mouse AA 1-224, full length Log in to see Polyclonal
0.12935057 ABIN525852 WB Rabbit AA 1-224, full length Log in to see Polyclonal
0.12935057 ABIN2155718 WB Goat Log in to see Polyclonal
0.12935057 ABIN4305319 WB Rabbit Log in to see Polyclonal
0.0011817255 ABIN357073 EIA IHC (p) WB Rabbit Ig AA 7-36, N-Term Log in to see Polyclonal 2
0.0011817255 ABIN357074 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
0.0011817255 ABIN374183 WB Goat C-Term Log in to see Polyclonal 1
0.0011817255 ABIN296980 WB Goat C-Term Log in to see Polyclonal
0.0011817255 ABIN1962695 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-DKK4 antibody for Western Blotting

Similar anti-DKK4 Antibodies

Application / Reactivity Human
ELISA 58 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Western Blotting (WB) 83 Antibodies

General

Antigen Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) Antibodies
Epitope C-Term
(53), (21), (16), (5), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(92), (19), (6), (2), (1), (1), (1), (1)
Host Goat
(87), (14), (3)
Clonality
Polyclonal
Conjugate This DKK4 antibody is un-conjugated
(8), (8), (7), (7), (7), (7)
Methode Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(89), (60), (29), (10), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-DKK4 Antibody

Target Details DKK4 Application Details Handling References for anti-DKK4 Antibody (ABIN184710) Images
Blocking Peptide Blocking peptide for this product available: ABIN370427
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence RQHARLRVCQKIEKL, from the C Terminus of the protein sequence according to NP_055235.1.

Target Details DKK4

Product Details anti-DKK4 Antibody Application Details Handling References for anti-DKK4 Antibody (ABIN184710) Images back to top
Antigen
Alternative Name DKK4 (DKK4 Antibody Abstract)
Background Synonyms: DKK4, dickkopf homolog 4 (Xenopus laevis), DKK-4, hdkk-4, Dickkopf gene 4, dickkopf (Xenopus laevis) homolog 4, MGC129562, MGC129563, dickkopf homolog 4
Gene ID 27121
NCBI Accession NP_055235
Research Area Stem Cells, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-DKK4 Antibody Target Details DKK4 Handling References for anti-DKK4 Antibody (ABIN184710) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30-35 kDa band seen in Jurkat lysate. Recommended for use at 1-3 µg/mL. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32 kDa species of DKK4.
Restrictions For Research Use only

Handling

Product Details anti-DKK4 Antibody Target Details DKK4 Application Details References for anti-DKK4 Antibody (ABIN184710) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-DKK4 Antibody (ABIN184710)

Product Details anti-DKK4 Antibody Target Details DKK4 Application Details Handling Images back to top
Background publications

Krupnik, Sharp, Jiang, Robison, Chickering, Amaravadi, Brown, Guyot, Mays, Leiby, Chang, Duong, Goodearl, Gearing, Sokol, McCarthy: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999

Images

Product Details anti-DKK4 Antibody Target Details DKK4 Application Details Handling References for anti-DKK4 Antibody (ABIN184710) back to top
Supplier Images
Western Blotting (WB) image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (C-Term) (ABIN184710) ABIN184710 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per la...