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anti-Mouse (Murine) DVL1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DVL1 Antibody (supplied by: Log in to see )

Antigen
Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) Antibodies
  • DSH
  • dsh1
  • DVL
  • Dvl
  • dvl-1
  • DVL-1
  • DVL-22
  • dvl1
  • DVL1
  • DVL1L1
  • DVL1P1
  • dvl2l
  • DVL4
  • mKIAA4029
  • Xdsh
Epitope
AA 20-70
17
14
12
10
10
3
3
3
3
3
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
54
33
21
12
5
2
2
1
1
1
1
1
1
Host
Rabbit
52
21
3
Clonality
Polyclonal
Conjugate
This DVL1 antibody is un-conjugated
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
61
43
16
10
8
6
5
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN670671
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008986177 ABIN670673 IHC (p) WB Biotin Rabbit IgG AA 20-70 Log in to see Polyclonal
0.0008986177 ABIN670680 IHC (p) WB HRP Rabbit IgG AA 20-70 Log in to see Polyclonal
0.0008986177 ABIN2962589 IHC (p) ELISA WB Goat AA 20-32, N-Term Log in to see Polyclonal

Top referenced anti-DVL1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-DVL1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 4 Antibodies 15 Antibodies 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 1 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 4 Antibodies 6 Antibodies
Western Blotting (WB) 12 Antibodies 23 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) Antibodies
Epitope AA 20-70
(17), (14), (12), (10), (10), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(54), (33), (21), (12), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(52), (21), (3)
Clonality
Polyclonal
Conjugate This DVL1 antibody is un-conjugated
(7), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(61), (43), (16), (10), (8), (6), (5), (1)
Pubmed 1 reference available
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Product Details anti-DVL1 Antibody

Target Details DVL1 Application Details Handling References for anti-DVL1 Antibody (ABIN670671) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human DVL1
Isotype IgG

Target Details DVL1

Product Details anti-DVL1 Antibody Application Details Handling References for anti-DVL1 Antibody (ABIN670671) Images back to top
Antigen
Alternative Name DVL1 (DVL1 Antibody Abstract)
Background

Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

Subcellular location: Cytoplasm, Cell membrane


Synonyms: DVL, DVL1L1, DVL1P1, Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1
Gene ID 1855
UniProt O14640
Pathways WNT Signaling

Application Details

Product Details anti-DVL1 Antibody Target Details DVL1 Handling References for anti-DVL1 Antibody (ABIN670671) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-DVL1 Antibody Target Details DVL1 Application Details References for anti-DVL1 Antibody (ABIN670671) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-DVL1 Antibody (ABIN670671)

Product Details anti-DVL1 Antibody Target Details DVL1 Application Details Handling Images back to top
Product cited in:

Hua, Xu, He, Jiang, Ye, Pan: "Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8421-31, 2015

Images

Product Details anti-DVL1 Antibody Target Details DVL1 Application Details Handling References for anti-DVL1 Antibody (ABIN670671) back to top
Supplier Images
SDS-PAGE (SDS) image for anti-DVL1 antibody (Dishevelled, Dsh Homolog 1 (Drosophila)) (AA 20-70) (ABIN670671) Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled ...
Immunohistochemistry (IHC) image for anti-DVL1 antibody (Dishevelled, Dsh Homolog 1 (Drosophila)) (AA 20-70) (ABIN670671) Images provided the Independent Validation Program (badge number 029657)Formalin-fixe...
'Independent Validation' Badge
Lot Number 140106
Method validated Immunohistochemistry
Positive Control Human kidney tubules
Negative Control Human colon stromal tissue
Notes While faint, signal is detectable in positive control tissue and not in negative control tissue. Note that staining is expected in colon epithelial cells, and is not expected in kidney glomeruli.
Primary Antibody
  • Antigen: Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1)
  • Catalog number: ABIN670671
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 140106
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human colon tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 1: Human kidney tissue stained with anti-DVL1 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 2: Human colon tissue stained with anti-DVL1 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1) (AA 20-70) antibody (ABIN670671) Figure 4: Human kidney tissue stained with secondary only (brown) and counterstained ...