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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||12 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|Antigen||Frizzled Family Receptor 1 (Fzd1) Antibodies|
|Epitope||AA 369-400, C-Term Alternatives|
|Conjugate||This FZD1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FZD1 AntibodyTarget Details FZD1 Application Details Handling Images
|Sequence||YIAGFLLEDR VVCNDKFAED GARTVAQG|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Frizzled-1(FZD1) detection. Tested with WB, IHC-P in Human.
Gene Name: frizzled class receptor 1
Protein Name: Frizzled-1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1 (369-400aa YIAGFLLEDRVVCNDKFAEDGARTVAQG TKKE), identical to the related mouse and rat sequences.|
Target Details FZD1Product Details anti-FZD1 Antibody Application Details Handling Images back to top
|Alternative Name||FZD1 (Fzd1 Antibody Abstract)|
|Background||Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.|
Application DetailsProduct Details anti-FZD1 Antibody Target Details FZD1 Handling Images back to top
WB: Concentration: 0.1-0.6 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-2 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-FZD1 Antibody Target Details FZD1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-FZD1 Antibody Target Details FZD1 Application Details Handling back to top