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anti-Mouse (Murine) FZD3 antibody for Immunocytochemistry

Recommended FZD3 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 3 (FZD3) Antibodies
  • FZD3
  • fz3
  • Fz-3
  • Xfz3
  • frz3
  • hfz3
  • frz-3
  • frizzled3
  • frizzled-3
  • FZ-3
  • AU020229
  • D930050A07Rik
  • Fz3
  • frizzled family receptor 3
  • frizzled homolog 3 (Drosophila)
  • FZD3
  • fzd3
  • Fzd3
Epitope
Cysteine-Rich Domain, Extracellular
17
15
14
13
12
9
8
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
113
66
25
4
4
4
4
4
3
3
3
1
1
1
Host
Rat
80
17
15
7
Clonality
Monoclonal
Conjugate
This FZD3 antibody is conjugated to Biotin
6
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
64
56
20
17
15
14
13
2
2
2
1
1
1
Supplier
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Catalog No. ABIN470848
$ 837.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN301678 ICC IHC WB Goat IgG Cysteine-Rich Domain, Extracellular Log in to see Polyclonal

Similar anti-FZD3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 23 Antibodies 54 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies 13 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Western Blotting (WB) 34 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Frizzled Family Receptor 3 (FZD3) Antibodies
Epitope Cysteine-Rich Domain, Extracellular
(17), (15), (14), (13), (12), (9), (8), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(113), (66), (25), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1)
Host Rat
(80), (17), (15), (7)
Clonality
Monoclonal
Conjugate This FZD3 antibody is conjugated to Biotin
(6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(64), (56), (20), (17), (15), (14), (13), (2), (2), (2), (1), (1), (1)
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Product Details anti-FZD3 Antibody

Target Details FZD3 Application Details Handling Images
Specificity Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.
Purification Protein G purified
Immunogen NS0-derived rmFrizzled-3 extracellular CRD.
Isotype IgG2a

Target Details FZD3

Product Details anti-FZD3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FZD3 / Frizzled 3 (FZD3 Antibody Abstract)
Background Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3
Gene ID 7976
UniProt Q9NPG1
Pathways WNT Signaling

Application Details

Product Details anti-FZD3 Antibody Target Details FZD3 Handling Images back to top
Application Notes Approved: Flo, ICC, IHC-Fr, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 μg/mL to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-FZD3 Antibody Target Details FZD3 Application Details Images back to top
Format Lyophilized
Reconstitution 1 mL sterile PBS
Concentration Lot specific
Buffer Lyophilized from PBS, BSA
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment +4°C or -20°C, Avoid repeated freezing and thawing.