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anti-Human FZD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended FZD4 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • anon-WO0170980.10
  • anon-WO0170980.11
  • CD344
  • CG4626
  • DFz4
  • Dfz4
  • Dmel\\CG4626
  • Dm Fz4
  • EVR1
  • FEVR
  • frizzled4
  • FZ-4
  • Fz-4
  • fz1
  • fz4
  • Fz4
  • FZD4S
  • FzE4
  • GPCR
  • hFz4
  • zg01
Reactivity
Human
108
57
44
4
3
3
2
2
2
2
2
2
1
1
1
Host
Mouse
77
23
10
7
Clonality
Monoclonal
Conjugate
This FZD4 antibody is un-conjugated
7
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
68
53
13
13
12
12
11
3
2
1
Supplier
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Catalog No. ABIN2689205
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Top referenced anti-FZD4 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-FZD4 Antibodies

Application / Reactivity Human
ELISA 51 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Western Blotting (WB) 61 Antibodies

General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Reactivity Human
(108), (57), (44), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(77), (23), (10), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FZD4 antibody is un-conjugated
(7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
(68), (53), (13), (13), (12), (12), (11), (3), (2), (1)
Pubmed 8 references available
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Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling References for anti-FZD4 Antibody (ABIN2689205) Images
Brand BD Pharmingen™
Characteristics The CH3A4A7 monoclonal antibody reacts with an extracellular epitope on human CD344 which is also known as Frizzled family receptor 4 (FZD4) or Frizzled-4. CD344 is a member of the Frizzled family of proteins within the G protein-coupled receptor (GPCR) superfamily. CD344 is a receptor for Wnt proteins and participates in the activation of the Wnt/beta-catenin pathway (canonical Wnt signaling). Patients with familial exudative vitreoretinopathy (FEVR) carry mutations in Frizzled-4. This disease is characterized by retinal degeneration and hearing loss. On tissues, CD344 is almost ubiquitously expressed. CD344 is highly expressed in adult heart and skeletal muscle. Flow Cytometric and Immunohistochemical Analysis of CD344 Expression. Panel A: Flow cytometric analysis of CD344 expression on human cell lines. Cells from the Weri-RB-1 Retinoblastoma Cell Line (ATCC HTB-169, solid line histogram) and Daudi Burkitt's Lymphoma Cell Line (ATCC CCL-213, dashed line histogram) were stained with Purified Mouse Anti-Human CD344 antibody (Cat. No. 563657). The cells were then washed and stained with PE Polyclonal Goat Anti-Mouse Ig (Cat. No.550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Panel B: Immunohistochemical analysis of CD344 expression by cardiac muscle. Acetone-fixed, frozen human heart sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, Left Image) or Purified Mouse Anti-Human CD344 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:40x.

BD Pharmingen™ Purified Mouse Anti-Human CD344 - Purified - Clone CH3A4A7 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human neuroblastoma Cell Line
Clone CH3A4A7
Isotype IgG1, kappa

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling References for anti-FZD4 Antibody (ABIN2689205) Images back to top
Antigen
Alternative Name CD344 (FZD4 Antibody Abstract)
Background Synonyms: FZD4, Frizzled family receptor 4, Frizzled-4, Fz-4, FzE4, hFz4, EVR1, FEVR
Pathways WNT Signaling

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling References for anti-FZD4 Antibody (ABIN2689205) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details References for anti-FZD4 Antibody (ABIN2689205) Images back to top
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-FZD4 Antibody (ABIN2689205)

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling Images back to top
Product cited in:

Wirths, Malenke, Kluba, Rieger, Müller, Schleicher, Hann von Weyhern, Nagl, Fend, Vogel, Mayer, Kanz, Bühring, Kopp: "Shared cell surface marker expression in mesenchymal stem cells and adult sarcomas." in: Stem cells translational medicine, Vol. 2, Issue 1, pp. 53-60, 2013

Ells, Guernsey, Wallace, Zheng, Vincer, Allen, Ingram, DaSilva, Siebert, Sheidow, Beis, Robitaille: "Severe retinopathy of prematurity associated with FZD4 mutations." in: Ophthalmic genetics, Vol. 31, Issue 1, pp. 37-43, 2010

Nikopoulos, Venselaar, Collin, Riveiro-Alvarez, Boonstra, Hooymans, Mukhopadhyay, Shears, van Bers, de Wijs, van Essen, Sijmons, Tilanus, van Nouhuys, Ayuso, Hoefsloot, Cremers: "Overview of the mutation spectrum in familial exudative vitreoretinopathy and Norrie disease with identification of 21 novel variants in FZD4, LRP5, and NDP." in: Human mutation, Vol. 31, Issue 6, pp. 656-66, 2010

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, 2007

Gregorieff, Pinto, Begthel, Destrée, Kielman, Clevers: "Expression pattern of Wnt signaling components in the adult intestine." in: Gastroenterology, Vol. 129, Issue 2, pp. 626-38, 2005

MacDonald, Goldberg, Macfarlane, Samuels, Trese, Shastry: "Genetic variants of frizzled-4 gene in familial exudative vitreoretinopathy and advanced retinopathy of prematurity." in: Clinical genetics, Vol. 67, Issue 4, pp. 363-6, 2005

Xu, Wang, Dabdoub, Smallwood, Williams, Woods, Kelley, Jiang, Tasman, Zhang, Nathans: "Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair." in: Cell, Vol. 116, Issue 6, pp. 883-95, 2004

Kirikoshi, Sagara, Koike, Tanaka, Sekihara, Hirai, Katoh: "Molecular cloning and characterization of human Frizzled-4 on chromosome 11q14-q21." in: Biochemical and biophysical research communications, Vol. 264, Issue 3, pp. 955-61, 1999