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anti-Rat (Rattus) FZD7 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended FZD7 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 7 (FZD7) Antibodies
  • cFz-7
  • frizzled-7
  • frizzled7
  • frz-7
  • frz7
  • Fz-7
  • Fz7
  • fz7
  • fzd7-a
  • fzd7-b
  • FzE3
  • fze3
  • Xfz7
Epitope
AA 550-574
29
15
14
12
11
9
6
5
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
106
52
22
5
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
80
24
11
7
Clonality
Polyclonal
Conjugate
This FZD7 antibody is un-conjugated
8
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
81
70
20
19
11
7
4
2
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN710051
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088400714 ABIN2813396 IF (p) Alexa Fluor 594 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN897666 IF (p) Alexa Fluor 555 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN897669 IF (p) Alexa Fluor 350 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN897665 IF (p) Alexa Fluor 488 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN897667 IF (p) Alexa Fluor 647 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN710054 IF (p) Cy3 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN710057 IF (p) Cy7 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN710058 IF (p) FITC Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN710056 IF (p) Cy5.5 Rabbit IgG AA 550-574 Log in to see Polyclonal
0.00088400714 ABIN710055 IF (p) Cy5 Rabbit IgG AA 550-574 Log in to see Polyclonal

Top referenced anti-FZD7 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-FZD7 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 6 Antibodies 22 Antibodies 60 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 8 Antibodies
Western Blotting (WB) 10 Antibodies 32 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 4 Antibodies 8 Antibodies

General

Antigen Frizzled Family Receptor 7 (FZD7) Antibodies
Epitope AA 550-574
(29), (15), (14), (12), (11), (9), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(106), (52), (22), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(80), (24), (11), (7)
Clonality
Polyclonal
Conjugate This FZD7 antibody is un-conjugated
(8), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(81), (70), (20), (19), (11), (7), (4), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-FZD7 Antibody

Target Details FZD7 Application Details Handling References for anti-FZD7 Antibody (ABIN710051) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Frizzled 7
Isotype IgG

Target Details FZD7

Product Details anti-FZD7 Antibody Application Details Handling References for anti-FZD7 Antibody (ABIN710051) Images back to top
Antigen
Alternative Name Frizzled 7 (FZD7 Antibody Abstract)
Background

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Subcellular location: Cytoplasm


Synonyms: FzE3, Frizzled-7, Fz-7, hFz7, FZD7
Gene ID 8324
UniProt O75084
Research Area Cancer, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-FZD7 Antibody Target Details FZD7 Handling References for anti-FZD7 Antibody (ABIN710051) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-FZD7 Antibody Target Details FZD7 Application Details References for anti-FZD7 Antibody (ABIN710051) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-FZD7 Antibody (ABIN710051)

Product Details anti-FZD7 Antibody Target Details FZD7 Application Details Handling Images back to top
Product cited in:

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, 2015

Images

Product Details anti-FZD7 Antibody Target Details FZD7 Application Details Handling References for anti-FZD7 Antibody (ABIN710051) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-FZD7 antibody (Frizzled Family Receptor 7) (AA 550-574) (ABIN710051) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled ...
Immunohistochemistry (IHC) image for anti-FZD7 antibody (Frizzled Family Receptor 7) (AA 550-574) (ABIN710051) Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled...
'Independent Validation' Badge
Lot Number 120426
Method validated Western Blotting
Positive Control MDA-MB-231 cells
Negative Control C6/36 cells (non-reactive species)
Notes A strong band was observed in the positive control sample at the expected molecular weight of ~64 kDa. The band is also faintly observed in the negative control sample, which may indicate species cross-reactivity.
Primary Antibody
  • Antigen: Frizzled Family Receptor 7 (FZD7)
  • Catalog number: ABIN710051
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 120426
  • Antibody Dilution: 1:100
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: MDA-MB-231 cells
  • Negative control: C6/36 cells
Protocol
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 24 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 550-574) antibody (ABIN710051) Figure 1: Western Blot for FZD7. Arrowhead indicates the expected molecular weight of...