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anti-Dog (Canine) FZD8 antibody for Western Blotting

Recommended FZD8 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 8 (FZD8) Antibodies
  • FZ-8
  • FZD8
  • cFz-8
  • Fz-8
  • frizzled8
  • xfz8
  • Xfz8
  • frizzled-8
  • hfz8
  • hFZ8
  • fzd8
  • Fz8
  • frizzled family receptor 5
  • frizzled family receptor 8
  • frizzled homolog 8 (Drosophila)
  • FZD5
  • fzd8-b
  • FZD8
  • fzd8
  • Fzd8
  • fzd8-a
Epitope
N-Term
18
14
6
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
61
36
23
7
6
5
3
3
2
2
Host
Rabbit
44
21
4
1
Clonality
Polyclonal
Conjugate
This FZD8 antibody is un-conjugated
3
1
1
1
1
1
Application
Western Blotting (WB)
47
33
25
21
7
3
1
1
Options
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Catalog No. ABIN2776728
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870947 ABIN2562711 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN374634 IHC (p) WB Goat C-Term Log in to see Polyclonal
1 ABIN297075 ELISA WB Goat AA 684-694 Log in to see Polyclonal

Top referenced anti-FZD8 antibody for Western Blotting

Similar anti-FZD8 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Western Blotting (WB) 39 Antibodies 26 Antibodies 16 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 12 Antibodies 9 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 9 Antibodies 6 Antibodies 1 Antibodies
Immunofluorescence (IF) 7 Antibodies 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen Frizzled Family Receptor 8 (FZD8) Antibodies
Epitope N-Term
(18), (14), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(61), (36), (23), (7), (6), (5), (3), (3), (2), (2)
Host Rabbit
(44), (21), (4), (1)
Clonality
Polyclonal
Conjugate This FZD8 antibody is un-conjugated
(3), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(47), (33), (25), (21), (7), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-FZD8 Antibody

Target Details FZD8 Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) Images
Blocking Peptide Blocking peptide for this product available: ABIN977174
Sequence PDTLCMDYNR TDLTTAAPSP PRRLPPPPPG EQPPSGSGHG RPPGARPPHR
Predicted Reactivity Dog: 100%, Human: 100%, Mouse: 93%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against FZD8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human FZD8

Target Details FZD8

Product Details anti-FZD8 Antibody Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Antigen
Alternative Name FZD8 (FZD8 Antibody Abstract)
Background FZD8 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
Alias Symbols: FZ-8, hFZ8
Protein Interaction Partner: WNT1, LRP5, LRP6, SUMO1, GOPC, RYK,
Protein Size: 694
Molecular Weight 73 kDa
Gene ID 8325
NCBI Accession NM_031866, NP_114072
UniProt Q9H461
Pathways WNT Signaling

Application Details

Product Details anti-FZD8 Antibody Target Details FZD8 Handling References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 694 AA

Restrictions For Research Use only

Handling

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details References for anti-FZD8 Antibody (ABIN2776728) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD8 Antibody (ABIN2776728)

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details Handling Images back to top
Product cited in:

Yin, Xu, Bonfil, Banerjee, Sarkar, Sethi, Reddy: "Tumor-initiating cells and FZD8 play a major role in drug resistance in triple-negative breast cancer." in: Molecular cancer therapeutics, Vol. 12, Issue 4, pp. 491-8, 2013

Background publications

Lu, Yamamoto, Ortega, Baltimore: "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth." in: Cell, Vol. 119, Issue 1, pp. 97-108, 2004

Images

Product Details anti-FZD8 Antibody Target Details FZD8 Application Details Handling References for anti-FZD8 Antibody (ABIN2776728) back to top
Supplier Images
Western Blotting (WB) image for anti-FZD8 antibody (Frizzled Family Receptor 8) (N-Term) (ABIN2776728) WB Suggested Anti-FZD8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...