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anti-Monkey GPBAR1 antibody for Western Blotting

Recommended GPBAR1 Antibody (supplied by: Log in to see )

Antigen
G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) Antibodies
  • BG37
  • CD344
  • EVR1
  • FEVR
  • Fz-4
  • Fz4
  • FZD4S
  • FzE4
  • GPBAR1
  • Gpbar1
  • GPCR
  • GPCR19
  • GPR131
  • hFz4
  • M-BAR
  • MGC84403
  • Tgr5
  • TGR5
Epitope
AA 302-322
17
14
8
4
3
3
2
2
2
2
2
2
2
1
1
Reactivity
Chimpanzee, Horse (Equine), Human, Monkey, Mouse (Murine)
98
12
7
3
2
2
1
1
1
1
1
1
Host
Rabbit
90
9
Clonality
Polyclonal
Conjugate
This GPBAR1 antibody is un-conjugated
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
36
33
16
11
9
8
5
2
Supplier
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Available images

Catalog No. ABIN372269
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011731827 ABIN1740029 IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-GPBAR1 Antibodies

Application / Reactivity Human Chimpanzee Horse (Equine) Monkey Mouse (Murine)
Western Blotting (WB) 36 Antibodies 1 Antibodies 2 Antibodies 2 Antibodies 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 1 Antibodies 3 Antibodies 3 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunofluorescence (IF) 5 Antibodies
Flow Cytometry (FACS) 9 Antibodies
ELISA 8 Antibodies

General

Antigen G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) Antibodies
Epitope AA 302-322
(17), (14), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity Chimpanzee, Horse (Equine), Human, Monkey, Mouse (Murine)
(98), (12), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(90), (9)
Clonality
Polyclonal
Conjugate This GPBAR1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(36), (33), (16), (11), (9), (8), (5), (2)
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Product Details anti-GPBAR1 Antibody

Target Details GPBAR1 Application Details Handling Images
Specificity Amino acids 302-322 of Human BG37/TGR5
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee, Horse, Monkey and Mouse.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen Synthetic peptide (KLH conjugated) corresponding to a portion of the amino acids 302-322 of Human BG37/TGR5.
Isotype IgG

Target Details GPBAR1

Product Details anti-GPBAR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GPBAR1 / TGR5 (GPBAR1 Antibody Abstract)
Background GPBAR1/TGR5 is a member of the G protein-coupled receptor family (GPCRs, or GPRs) which contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR TGR5 is a receptor for bile acids. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. It may be involved in the suppression of macrophage functions by bile acids.Synonyms: BG37, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, M-BAR, Membrane-type receptor for bile acids
Gene ID 151306
NCBI Accession NP_001070659
UniProt Q8TDU6
Pathways WNT Signaling

Application Details

Product Details anti-GPBAR1 Antibody Target Details GPBAR1 Handling Images back to top
Application Notes Western Blot: 2-4 μg/mL. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. The stained slides were evaluated by apathologist to confirm staining specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GPBAR1 Antibody Target Details GPBAR1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % Sodium Azide as preservative and 0.2 % Gelatin as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Images

Product Details anti-GPBAR1 Antibody Target Details GPBAR1 Application Details Handling back to top
Supplier Images
 image for anti-GPBAR1 antibody (G Protein-Coupled Bile Acid Receptor 1) (AA 302-322) (ABIN372269) anti-G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (AA 302-322) antibody
 image for anti-GPBAR1 antibody (G Protein-Coupled Bile Acid Receptor 1) (AA 302-322) (ABIN372269) anti-G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (AA 302-322) antibody (Image 2)
 image for anti-GPBAR1 antibody (G Protein-Coupled Bile Acid Receptor 1) (AA 302-322) (ABIN372269) anti-G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (AA 302-322) antibody (Image 3)