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anti-Human LEF1 antibody for Enzyme Immunoassay

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
N-Term, AA 17-45
20
16
15
6
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
118
33
25
7
4
4
3
2
1
1
1
1
1
Host
Rabbit
53
45
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
108
62
20
12
6
4
3
2
2
1
1
1
1
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Catalog No. ABIN953158
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854307 ABIN126828 EIA WB Mouse IgG1 Log in to see 2D12

Top referenced anti-LEF1 antibody for Enzyme Immunoassay

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General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope N-Term, AA 17-45
(20), (16), (15), (6), (6), (6), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(118), (33), (25), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(53), (45), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(108), (62), (20), (12), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN953158) Images
Specificity This antibody recognizes Human LEF1 (N-term).
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human LEF1
Isotype Ig

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN953158) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Molecular Weight 44201 Da
Gene ID 51176
NCBI Accession NP_001124185
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN953158) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN953158) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-LEF1 Antibody (ABIN953158)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Gutierrez, Tschumper, Wu, Shanafelt, Eckel-Passow, Huddleston, Slager, Kay, Jelinek: "LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis." in: Blood, Vol. 116, Issue 16, pp. 2975-83, 2010

Chen, Zhang, Pincus, Wu, Ricks, Liu, Sun, Maclaren, Lan: "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 5047-54, 2010

Kalsi, Kuo, Aliev, Alexander, McMichael, Patterson, Walsh, Zhao, Schuckit, Nurnberger, Edenberg, Kramer, Hesselbrock, Tischfield, Vladimirov, Prescott, Dick, Kendler, Riley: "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2497-506, 2010

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN953158) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (N-Term) (ABIN953158) Western blot analysis of LEF1 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/...