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anti-Human LEF1 antibody for Flow Cytometry

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
Pro82
15
13
13
9
6
6
6
5
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
119
39
30
9
7
7
5
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
52
47
16
5
Clonality
Monoclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
108
64
20
13
7
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN221435
$ 683.83
Plus shipping costs $45.00

Similar anti-LEF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 20 Antibodies 20 Antibodies 64 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies 2 Antibodies
Western Blotting (WB) 25 Antibodies 34 Antibodies
Biochemical Assay (BCA) 2 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope Pro82
(15), (13), (13), (9), (6), (6), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(119), (39), (30), (9), (7), (7), (5), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(52), (47), (16), (5)
Clonality
Monoclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(108), (64), (20), (13), (7), (4), (2), (2), (2), (2), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Specificity Detects endogenous level of total human LEF1 protein. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. Species cross-reactivity: Mouse and rat.
Purification Tissue culture supernatant
Immunogen Synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro82 of human LEF1.

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
Gene ID 51176
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes Approved: Flo (1:200), ICC, IF, IP (1:50), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5 % BSA, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.Immunoprecipitation: 1:50Flow Cytometry: 1:200Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, less than 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.