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anti-Human LEF1 antibody for Immunofluorescence

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
HMG Binding Domain
21
16
16
6
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
117
32
25
7
4
4
3
2
1
1
1
1
1
Host
Mouse
53
44
16
5
Clonality
Monoclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
107
62
18
11
5
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN264381
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32098043 ABIN526526 IF ELISA WB Mouse IgG1 kappa AA 14-123, partial Log in to see 5A3
0.32098043 ABIN526527 IF ELISA WB Mouse IgG1 kappa AA 14-123, partial Log in to see 2C9
0.32098043 ABIN526529 IF ELISA WB Mouse IgG2a kappa AA 33-138, full length Log in to see 1A8
0.0010804428 ABIN655987 IF WB Rabbit Ig AA 10-37, N-Term Log in to see Polyclonal 4
0.0010804428 ABIN396506 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 5A3
0.0010804428 ABIN221435 ICC FACS IF IP WB Rabbit IgG Pro82 Log in to see
0.0010804428 ABIN395469 IF WB Mouse IgG1 kappa AA 14-124 Log in to see 2C9
0.0010804428 ABIN187648 IF WB Mouse IgG1 Log in to see REMB6
0.0010804428 ABIN187649 IF WB Mouse IgG1 Log in to see REMB1
0.0010804428 ABIN264379 IF IHC (p) WB Mouse IgG1 Log in to see REMB1
0.0010804428 ABIN265951 ICC IF WB Mouse IgG1 Log in to see REMB1
0.0010804428 ABIN1812487 IF WB Rabbit AA 17-45 Log in to see Polyclonal
0.0010804428 ABIN1909578 IF IP IHC (p) WB Rabbit IgG AA 349-399 Log in to see Polyclonal
0.0010804428 ABIN479089 IF WB Mouse IgG1 Log in to see
0.0010804428 ABIN2299288 IF ELISA WB Mouse IgG1 kappa AA 14-123 Log in to see 2C9
0.0010804428 ABIN2299286 IF ELISA WB Mouse IgG1 kappa AA 14-123 Log in to see 5A3
0.0010804428 ABIN3031647 IF ELISA WB Rabbit Ig Fraction AA 10-37 Log in to see Polyclonal
0.0010804428 ABIN2797583 IF WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.0010804428 ABIN2772693 ELISA (Capture) IF ELISA WB Mouse IgG1, kappa Log in to see

Top referenced anti-LEF1 antibody for Immunofluorescence

Similar anti-LEF1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 18 Antibodies 63 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 4 Antibodies
Immunofluorescence (IF) 2 Antibodies 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 8 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 5 Antibodies
Western Blotting (WB) 29 Antibodies
Biochemical Assay (BCA) 2 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunoprecipitation (IP) 3 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope HMG Binding Domain
(21), (16), (16), (6), (6), (6), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(117), (32), (25), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(53), (44), (16), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(107), (62), (18), (11), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN264381) Images
Specificity REMB6 recognizes the High Mobility Group DNA domain of LEF-1.
Characteristics Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha
Purification Purified
Immunogen Full-length recombinant LEF-1 protein. Genename: LEF1
Clone REMB6
Isotype IgG1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN264381) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Gene ID 51176
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN264381) Images back to top
Application Notes Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN264381) Images back to top
Buffer PBS, 0.08 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-LEF1 Antibody (ABIN264381)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Hsu, Galceran, Grosschedl: "Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4807-18, 1998

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN264381) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (HMG Binding Domain) (ABIN264381) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody
Immunofluorescence (IF) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (HMG Binding Domain) (ABIN264381) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody (Image 2)