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anti-Human LEF1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
pSer42
15
14
13
9
6
6
6
5
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
123
41
32
10
8
8
5
5
4
4
4
4
2
2
2
1
1
Host
Rabbit
54
48
17
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
112
65
21
13
6
5
3
2
2
1
1
1
1
1
Options
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Catalog No. ABIN1387756
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN735248 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 3

Top referenced anti-LEF1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-LEF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 21 Antibodies 21 Antibodies 65 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies 2 Antibodies
Western Blotting (WB) 27 Antibodies 36 Antibodies
Biochemical Assay (BCA) 2 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope pSer42
(15), (14), (13), (9), (6), (6), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(123), (41), (32), (10), (8), (8), (5), (5), (4), (4), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(54), (48), (17), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(112), (65), (21), (13), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN1387756) Images
Specificity This antibody will recognize LEF1(Ser40) in mouse and rat
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Isotype IgG

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN1387756) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Subcellular location: Nucleus


Synonyms: LEF-1, TCF10, TCF7L3, TCF1ALPHA, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Gene ID 51176
UniProt Q9UJU2
Research Area Wnt, Transcription Factors, Lineage Markers
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN1387756) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN1387756) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-LEF1 Antibody (ABIN1387756)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Product cited in:

Fu, Huang, Wang, Chen, Huang, Lu, Liang, Zhang: "Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development." in: International journal of molecular sciences, Vol. 17, Issue 5, 2016

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN1387756) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (pSer42) (ABIN1387756) Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-...