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anti-Mouse (Murine) LEF1 antibody for Immunohistochemistry

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
C-Term
21
16
16
6
6
6
6
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
118
32
24
6
3
3
2
2
1
1
Host
Rabbit
53
45
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
108
62
20
11
6
4
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2779620
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22915025 ABIN2428345 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN2421791 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011319318 ABIN793575 ICC IHC WB Mouse IgG1 Log in to see REMB6
0.0011319318 ABIN1584697 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011319318 ABIN1088252 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN1088251 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-LEF1 antibody for Immunohistochemistry

Similar anti-LEF1 Antibodies

Application / Reactivity Human Chicken Cow (Bovine) Dog (Canine) Mouse (Murine) Pig (Porcine) Rabbit Rat (Rattus) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 110 Antibodies 1 Antibodies 4 Antibodies 7 Antibodies 29 Antibodies 4 Antibodies 3 Antibodies 21 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 12 Antibodies 1 Antibodies 1 Antibodies 3 Antibodies 8 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope C-Term
(21), (16), (16), (6), (6), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(118), (32), (24), (6), (3), (3), (2), (2), (1), (1)
Host Rabbit
(53), (45), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(108), (62), (20), (11), (6), (4), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2779620) Images
Sequence VKPQHEQRKE QEPKRPHIKK PLNAFMLYMK EMRANVVAEC TLKESAAINQ
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human LEF1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN2779620) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1.
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NP_057353, NM_016269
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN2779620) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 399 AA

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN2779620) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-LEF1 Antibody (ABIN2779620)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Liu, Driskell, Luo, Abbott, Filali, Cheng, Sigmund, Engelhardt: "Characterization of Lef-1 promoter segments that facilitate inductive developmental expression in skin." in: The Journal of investigative dermatology, Vol. 123, Issue 2, pp. 264-74, 2004

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN2779620) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (C-Term) (ABIN2779620) WB Suggested Anti-LEF1 Antibody Titration: 2.0ug/ml
Positive Control:
Immunohistochemistry (IHC) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (C-Term) (ABIN2779620) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (C-Term) antibody (Image 2)