anti-Pig (Porcine) LEF1 antibody for Western Blotting

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
Middle Region
15
13
13
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
128
40
30
10
6
6
5
4
4
4
3
3
3
2
1
1
Host
Rabbit
56
52
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
115
67
22
17
10
7
5
3
2
2
2
1
1
1
1
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Catalog No. ABIN2779621
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN464193 WB Rabbit IgG AA 72-121 Log in to see Polyclonal
1 ABIN202428 IHC (p) WB Rabbit IgG AA 282-331 Log in to see Polyclonal
1 ABIN297259 ELISA WB Goat AA 257-270 Log in to see Polyclonal
1 ABIN1909577 IP WB Rabbit IgG AA 250-300 Log in to see Polyclonal

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope Middle Region
(15), (13), (13), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(128), (40), (30), (10), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1)
Host Rabbit
(56), (52), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(115), (67), (22), (17), (10), (7), (5), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN979264
Sequence ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG
Predicted Reactivity Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 79%
Characteristics This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LEF1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1).
Alias Symbols: DKFZp586H0919, FLJ46390, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NM_016269, NP_057353
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 399 AA

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Middle Region) antibody (ABIN2779621) WB Suggested Anti-LEF1 Antibody Titration: 0.1-2.0ug/ml
Positive Control...
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Middle Region) antibody (ABIN2779621) Host: Rabbit
Target Name: LEF1
Sample Tissue: Human Fetal Heart
...