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anti-Human LRP6 antibody for ELISA

Recommended LRP6 Antibody (supplied by: Log in to see )

Antigen
Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) Antibodies
  • ADCAD2
  • adcad2
  • C030016K15Rik
  • Cd
  • Gw
  • LRP6
  • lrp6
  • lrp6-A
  • Xlrp6
Epitope
Internal Region
14
13
13
9
8
7
5
3
1
1
1
1
Reactivity
Human
78
19
5
3
1
1
1
1
1
Host
Goat
58
13
7
1
Clonality
Polyclonal
Conjugate
This LRP6 antibody is un-conjugated
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
32
31
29
20
12
11
8
3
2
1
1
1
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Catalog No. ABIN185307
$ 318.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012121415 ABIN250317 IHC (p) IHC ELISA Goat IgG AA 1546-1560 Log in to see Polyclonal 2
0.0012121415 ABIN291653 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
0.0012121415 ABIN2465909 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012121415 ABIN213979 IHC (p) ELISA Rabbit AA 1546-1560 Log in to see Polyclonal
0.0012121415 ABIN2469646 IHC ELISA Rabbit AA 1546-1560 Log in to see Polyclonal
0.0012121415 ABIN1910472 IHC ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1910473 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1910474 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1910470 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1910471 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN609034 IHC (p) ELISA Rabbit IgG AA 1546-1560 Log in to see Polyclonal
0.0012121415 ABIN2613300 ELISA Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1859708 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 981-1213 Log in to see Polyclonal
0.0012121415 ABIN1910475 IHC ELISA WB HRP Rabbit IgG C-Term Log in to see Polyclonal
0.0012121415 ABIN1736559 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
0.0012121415 ABIN1736560 IHC (p) ELISA Rabbit IgG AA 1546-1560 Log in to see Polyclonal
0.0012121415 ABIN2624976 ELISA WB Biotin Rabbit IgG AA 981-1213 Log in to see Polyclonal
0.0012121415 ABIN2613298 ELISA Rabbit IgG AA 981-1213 Log in to see Polyclonal
0.0012121415 ABIN2444997 IHC (fp) ELISA Goat IgG Internal Region Log in to see Polyclonal
0.0012121415 ABIN2475360 IHC (p) ELISA Goat IgG Internal Region Log in to see Polyclonal

Top referenced anti-LRP6 antibody for ELISA

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Application / Reactivity Human
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Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
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Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 33 Antibodies

General

Antigen Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) Antibodies
Epitope Internal Region
(14), (13), (13), (9), (8), (7), (5), (3), (1), (1), (1), (1)
Reactivity Human
(78), (19), (5), (3), (1), (1), (1), (1), (1)
Host Goat
(58), (13), (7), (1)
Clonality
Polyclonal
Conjugate This LRP6 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(32), (31), (29), (20), (12), (11), (8), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-LRP6 Antibody

Target Details LRP6 Application Details Handling References for anti-LRP6 Antibody (ABIN185307) Images
Blocking Peptide Blocking peptide for this product available: ABIN369437
Specificity This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence CMTSVATAKGYTSDL, from the internal region of the protein sequence according to NP_002327.2.

Target Details LRP6

Product Details anti-LRP6 Antibody Application Details Handling References for anti-LRP6 Antibody (ABIN185307) Images back to top
Antigen
Alternative Name LRP6 (LRP6 Antibody Abstract)
Background Synonyms: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6
Gene ID 4040, 16974
NCBI Accession NP_002327
Research Area Stem Cells, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-LRP6 Antibody Target Details LRP6 Handling References for anti-LRP6 Antibody (ABIN185307) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 35 kDa in Human Adipose lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180 kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-LRP6 Antibody Target Details LRP6 Application Details References for anti-LRP6 Antibody (ABIN185307) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-LRP6 Antibody (ABIN185307)

Product Details anti-LRP6 Antibody Target Details LRP6 Application Details Handling Images back to top
Background publications

Giles, van Es, Clevers: "Caught up in a Wnt storm: Wnt signaling in cancer." in: Biochimica et biophysica acta, Vol. 1653, Issue 1, pp. 1-24, 2003

Tamai, Semenov, Kato, Spokony, Liu, Katsuyama, Hess, Saint-Jeannet, He: "LDL-receptor-related proteins in Wnt signal transduction." in: Nature, Vol. 407, Issue 6803, pp. 530-5, 2000

Images

Product Details anti-LRP6 Antibody Target Details LRP6 Application Details Handling References for anti-LRP6 Antibody (ABIN185307) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-LRP6 antibody (Low Density Lipoprotein Receptor-Related Protein 6) (Internal Region) (ABIN185307) ABIN185307 (5µg/ml) staining of paraffin embedded human liver. Steamed antigen retrie...