You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Phospholipase C beta 4 antibody for Western Blotting

Recommended Phospholipase C beta 4 Antibody (supplied by: Log in to see )

Antigen
Phospholipase C beta 4 (PLCb4) Antibodies
  • A930039J07Rik
  • AI854601
  • ARCND2
  • Beta4aa
  • C230058B11Rik
  • PI-PLC
  • RATBETA4AA
Epitope
AA 752-961
1
1
Reactivity
Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
3
2
1
1
Host
Mouse
2
1
1
Clonality
Monoclonal
Application
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
4
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN968583
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13078709 ABIN968900 IF WB Mouse IgG1 AA 752-961 Log in to see 56-Phospholipase Cbeta4 4
0.0011727312 ABIN2149229 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011727312 ABIN1856286 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-Phospholipase C beta 4 antibody for Western Blotting

Similar anti-Phospholipase C beta 4 Antibodies

Application / Reactivity Fly (Calliphora) Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 2 Antibodies 4 Antibodies 3 Antibodies 2 Antibodies
Immunofluorescence (IF) 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
ELISA 2 Antibodies
BioImaging (BI) 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Phospholipase C beta 4 (PLCb4) Antibodies
Epitope AA 752-961
(1), (1)
Reactivity Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
(3), (2), (1), (1)
Host Mouse
(2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Application BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
(4), (2), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-Phospholipase C beta 4 Antibody

Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 Antibody (ABIN968583) Images
Cross-Reactivity Rat (Rattus), Mouse (Murine), Fruit Fly (Drosophila melanogaster)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Phospholipase Cbeta4 aa. 752-961
Clone 56-Phospholipase Cbeta4
Isotype IgG1

Target Details Phospholipase C beta 4

Product Details anti-Phospholipase C beta 4 Antibody Application Details Handling References for anti-Phospholipase C beta 4 Antibody (ABIN968583) Images back to top
Antigen
Alternative Name Phospholipase C beta 4 (PLCb4 Antibody Abstract)
Background Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
Synonyms: PLCbeta4
Molecular Weight 130 kDa
Pathways WNT Signaling

Application Details

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Handling References for anti-Phospholipase C beta 4 Antibody (ABIN968583) Images back to top
Application Notes Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details References for anti-Phospholipase C beta 4 Antibody (ABIN968583) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Phospholipase C beta 4 Antibody (ABIN968583)

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling Images back to top
Product cited in:

Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, 1999

Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, 1997

Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, 1996

Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, 1993

Images

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 Antibody (ABIN968583) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase C beta 4 antibody (PLCb4) (AA 752-961) (ABIN968583) Western blot analysis of Phospholipase Cbeta4 on a rat pituitary lysate (left). Lane ...
Immunofluorescence (IF) image for anti-Phospholipase C beta 4 antibody (PLCb4) (AA 752-961) (ABIN968583) Immunofluorescent staining of C6 cells (Rat glioma, ATCC CCL-107) (right). Cells were...
Immunofluorescence (IF) image for anti-Phospholipase C beta 4 antibody (PLCb4) (AA 752-961) (ABIN968583) anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (Image 3)
Western Blotting (WB) image for anti-Phospholipase C beta 4 antibody (PLCb4) (AA 752-961) (ABIN968583) anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (Image 4)