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|Antigen||Phospholipase C beta 4 (PLCb4) Antibodies|
|Epitope||AA 752-961 Alternatives|
|Reactivity||Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
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Product Details anti-Phospholipase C beta 4 AntibodyTarget Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images
|Cross-Reactivity||Fruit Fly (Drosophila melanogaster), Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Phospholipase Cbeta4 aa. 752-961|
Target Details Phospholipase C beta 4Product Details anti-Phospholipase C beta 4 Antibody Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
|Alternative Name||Phospholipase C beta 4 (PLCb4 Antibody Abstract)|
Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
|Molecular Weight||130 kDa|
|Pathways||WNT Signaling, Thyroid Hormone Synthesis, G-protein mediated Events|
Application DetailsProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
|Application Notes||The Drosophila melanogaster gene, no receptor potential A (abbreviated as norpA), encodes a 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase involved in calcium-mediated signaling, which is a component of the membrane fraction. Its amino acid sequence contains a phosphatidylinositol-specific phospholipase C (X domain), a phosphoinositide-specific phospholipase C (PLC) and a phosphatidylinositol-specific phospholipase C (Y domain). Similar sequences have been identified in C. elegans, Homo sapiens, Mus musculus, Rattus norvegicus, and Saccharomyces cerevisiae. It has been mapped by recombination to 1-6.5 and cytologically to 4C1.|
Related Products: ABIN967389, ABIN968539, ABIN968583
|Restrictions||For Research Use only|
HandlingProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-Phospholipase C beta 4 antibody (ABIN968900)Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling Images back to top
|Product cited in:||
Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, 1999
Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, 1997
Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, 1996
Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, 1993
ImagesProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) back to top