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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||31 Antibodies||36 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||11 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies||40 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Western Blotting (WB)||68 Antibodies|
|Antigen||Protein Kinase C, alpha (PKCa) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PKC alpha antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-PKC alpha AntibodyTarget Details PKC alpha Application Details Handling Images
|Specificity||The antibody only detects PKC alpha phosphorylated on Threonine 638.|
|Cross-Reactivity (Details)||Predicted to work with: Chicken, Bovine.|
|Purification||Protein A purified|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKCa was used as immunogen.|
Target Details PKC alphaProduct Details anti-PKC alpha Antibody Application Details Handling Images back to top
|Alternative Name||PRKCA (PKCa Antibody Abstract)|
Activation of PKC plays a crucial role in tranducing signals for a variety of cellularresponses, including tumor proliferation (1). PKC isoforms have been classified intothree groups: classical, novel, and atypical. Members of these three PKC groups containan autoinhibitory domain that binds to the substrate binding site in the catalytic domain,preventing its activation in the absence of cofactors or activators (2,3). The activity ofPKC is under the control of three distinct phosphorylation events, including that of theThr641 autophosphorylation site (4). Alternate Names: anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody,anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-ProteinKinase C alpha antibody, anti-Protein kinase C alpha type antibody.
Gene Symbol: PRKCA
|Gene ID||5578, 18750, 25636|
|Pathways||WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 80kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:40, Western Blot 1:20000
Protocol specific for PKC alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top