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anti-Human PKC alpha antibody for Immunocytochemistry

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
Thr638
50
34
17
15
15
13
12
11
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
253
138
114
23
19
19
16
16
16
3
3
2
1
1
Host
Rabbit
223
42
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
7
7
5
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
206
123
109
54
49
37
36
22
21
20
15
5
4
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN189677
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN189676 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y124
0.0009965416 ABIN189675 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y143
0.0009965416 ABIN220833 ICC FACS IF IP WB Rabbit Log in to see Polyclonal
0.0009965416 ABIN408073 ICC IF IHC (p) IHC WB Rabbit IgG Thr495 Log in to see Polyclonal
0.0009965416 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345806 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009965416 ABIN4345813 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA DyLight 405 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345814 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 350 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345815 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB PerCP Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345816 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Allophycocyanin Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345817 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 755 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345818 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 680 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345819 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 488 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345820 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 405LS Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345821 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Alexa Fluor 405 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345822 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Alexa Fluor 488 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345823 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Alexa Fluor 647 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN4345824 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Alexa Fluor 700 Mouse IgG2a Log in to see MC5
0.0009965416 ABIN2743806 ICC Rabbit IgG Log in to see Polyclonal
0.0009965416 ABIN4345807 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 47 Antibodies 52 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 17 Antibodies 26 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 20 Antibodies 20 Antibodies
Immunofluorescence (IF) 23 Antibodies 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 87 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope Thr638
(50), (34), (17), (15), (15), (13), (12), (11), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(253), (138), (114), (23), (19), (19), (16), (16), (16), (3), (3), (2), (1), (1)
Host Rabbit
(223), (42), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (7), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(206), (123), (109), (54), (49), (37), (36), (22), (21), (20), (15), (5), (4), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity The antibody only detects PKC alpha phosphorylated on Threonine 638.
Cross-Reactivity (Details) Predicted to work with: Chicken, Bovine.
Purification Protein A purified
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKCa was used as immunogen.
Clone E195
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Activation of PKC plays a crucial role in tranducing signals for a variety of cellularresponses, including tumor proliferation (1). PKC isoforms have been classified intothree groups: classical, novel, and atypical. Members of these three PKC groups containan autoinhibitory domain that binds to the substrate binding site in the catalytic domain,preventing its activation in the absence of cofactors or activators (2,3). The activity ofPKC is under the control of three distinct phosphorylation events, including that of theThr641 autophosphorylation site (4). Alternate Names: anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody,anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-ProteinKinase C alpha antibody, anti-Protein kinase C alpha type antibody.
Gene Symbol: PRKCA
Gene ID 5578, 18750, 25636
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 80kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:40, Western Blot 1:20000
Protocol Protocol specific for PKC alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) Western blot analysis on 293 cell lysate using anti-Phospho-PKCa (pT638) RabMAb (cat....
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-em...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (Thr638) (ABIN189677) anti-Protein Kinase C, alpha (PKCa) (Thr638) antibody (Image 3)