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anti-Human PKC alpha antibody for Immunohistochemistry

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
pThr638
49
34
17
15
15
13
11
9
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
228
114
91
8
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
213
27
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
185
106
89
35
33
22
22
20
6
5
4
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN1531462
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22053991 ABIN189677 ICC IF IHC (p) IP IHC WB Rabbit IgG Thr638 Log in to see E195
0.22053991 ABIN1532477 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN1533205 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN189676 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y124
0.22053991 ABIN189675 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y143
0.22053991 ABIN1874290 IHC WB Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN2470413 IHC ELISA Rabbit AA 491-501 Log in to see Polyclonal
0.22053991 ABIN2499398 IHC ELISA WB Rabbit IgG Thr638 Log in to see Polyclonal
0.22053991 ABIN2499395 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN2495480 FACS IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.22053991 ABIN2495481 FACS IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.22053991 ABIN2403540 IHC WB Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN2428595 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22053991 ABIN2422039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011363331 ABIN967750 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 6
0.0011363331 ABIN967751 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 5
0.0011363331 ABIN1585060 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011363331 ABIN1926570 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
0.0011363331 ABIN1926572 IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
0.0011363331 ABIN1926571 IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for Immunohistochemistry

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 7 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope pThr638
(49), (34), (17), (15), (15), (13), (11), (9), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(228), (114), (91), (8), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(213), (27), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(185), (106), (89), (35), (33), (22), (22), (20), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) Images
Specificity PKC alpha (Phospho-Thr638) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr638.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638.
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Antigen
Alternative Name PKC alpha (PKCa Antibody Abstract)
Background Molecular weight of target protein: 76 kDa
Synonyms: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C, alpha type
Molecular Weight 76 kDa
Gene ID 5578
OMIM 176960
UniProt P17252
Research Area Cardiovascular, Hypertrophy, Signaling, Protein Modifications, Kinases/Phosphatases
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.531704, Hs.708867 (NCBI Gene Symbol: PRKCA)

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-PKC alpha Antibody (ABIN1531462)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, 2015

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (pThr638) (ABIN1531462) Western blot analysis of extracts from NIH-3T3 cells treated with UV 15', using PKC a...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (pThr638) (ABIN1531462) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC ...