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anti-Mammalian PKC alpha antibody for Immunohistochemistry (Frozen Sections)

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Reactivity
Human, Mammalian
228
115
92
8
4
4
3
3
1
1
1
1
1
1
Host
Mouse
214
26
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
10
9
7
6
5
5
4
4
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
185
107
90
35
33
22
22
20
5
5
4
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN119008
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088050903 ABIN2476256 IHC (fro) WB Mouse IgG2a Log in to see 133 1

Top referenced anti-PKC alpha antibody for Immunohistochemistry (Frozen Sections)

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General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Reactivity Human, Mammalian
(228), (115), (92), (8), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(214), (26), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(10), (9), (7), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(185), (107), (90), (35), (33), (22), (22), (20), (5), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity PKC alpha antibody (Cat#SM1436) recognises the alpha isoform of Protein Kinase C. It recognises the sequence PQFVHPILQSAV at the C terminus of PKCa.
Purification Supernatant
Immunogen Protein Kinase C alpha (PKC alpha). Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone 133
Isotype IgG2a

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Research Area Cardiovascular, Hypertrophy, Kinases/Phosphatases
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Immunohistochemistry on frozen sections: Neat - 1/10. Western Blot: 1/10 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.