anti-Mouse (Murine) PKC alpha antibody for Enzyme Immunoassay

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Epitope
N-Term
45
26
18
17
16
15
15
12
7
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
272
194
153
33
29
27
21
21
20
5
4
3
3
3
2
1
1
1
Host
Rabbit
233
47
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
11
9
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
226
124
98
69
50
43
27
26
24
23
5
4
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN453789
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN272065 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope N-Term
(45), (26), (18), (17), (16), (15), (15), (12), (7), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(272), (194), (153), (33), (29), (27), (21), (21), (20), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(233), (47), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (11), (9), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(226), (124), (98), (69), (50), (43), (27), (26), (24), (23), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN453789) Images
Specificity This antibody reacts to PRKCA.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human PRKCA

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN453789) Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN453789) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN453789) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PKC alpha antibody (ABIN453789)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Background publications

Gysin, Imber: "Replacement of Ser657 of protein kinase C-alpha by alanine leads to premature down regulation after phorbol-ester-induced translocation to the membrane." in: European journal of biochemistry / FEBS, Vol. 240, Issue 3, pp. 747-50, 1996

Rider, Vandamme, Lebeau, Vertommen, Vidal, Rousseau, Vandekerckhove, Hue: "The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 405-11, 1992

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN453789) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody (ABIN453789) Western blot analysis of PRKCA Antibody (N-term) in NIH-3T3 cell line lysates (35ug/l...