anti-Rabbit PKC alpha antibody for Functional Studies

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
272
194
152
32
29
26
21
21
20
5
4
3
3
3
2
1
1
1
Host
Mouse
234
46
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
11
9
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
226
124
98
69
50
43
26
26
24
23
5
4
4
3
2
1
1
1
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Catalog No. ABIN199577
$ 837.83
Plus shipping costs $45.00

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(272), (194), (152), (32), (29), (26), (21), (21), (20), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(234), (46), (2)
Clonality
Monoclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (11), (9), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
(226), (124), (98), (69), (50), (43), (26), (26), (24), (23), (5), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity Recognizes rabbit PKC-a at ~82kD. Species cross-reactivity: Rat, human, bovine and mouse.
Purification Protein G purified
Immunogen Purified PKC-a from rabbit brain cytosol.

Type of Immunogen: Purified protein
Isotype IgG1

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA / PKC-Alpha (PKCa Antibody Abstract)
Background Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha
Gene ID 5578
UniProt P17252
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Approved: Func, IP, Neut, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Neutralization. Western Blot: 0.5-2 μg/mL. Detects PKC-a (~82kD) in human A431 carcinoma cells and a bovine brain cytosolic extract. Immunoprecipitation: 4 μg. Immunoprecipitated PKC-a from an A431 RIPA cell lysate and a bovine brain extract. Neutralization: 20 μg of the antibody can inhibit >50 % of the kinase activity of 20 ng Type III PKC.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.