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anti-Rat (Rattus) PKC alpha antibody for Enzyme Immunoassay

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Reactivity
Human, Mouse (Murine), Rat (Rattus)
265
192
151
30
26
24
18
18
17
6
4
3
3
3
2
1
1
1
1
Host
Rabbit
228
45
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
8
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
218
122
99
57
50
38
28
26
21
19
5
4
3
3
2
1
1
1
1
1
1
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Catalog No. ABIN272065
$ 390.50
Plus shipping costs $45.00

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 56 Antibodies 75 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 19 Antibodies 35 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(265), (192), (151), (30), (26), (24), (18), (18), (17), (6), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(228), (45), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (8), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(218), (122), (99), (57), (50), (38), (28), (26), (21), (19), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity This antibody detects endogenous levels of PKC α, β I, II, γ, δ, ε, η, ζ and θ isoforms. (region surrounding Thr495)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.Synonyms: Protein kinase C
Molecular Weight approx. 75-88 kDa
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Research Area Cardiovascular, Hypertrophy, Kinases/Phosphatases
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes ELISA: 1: 5000approx. 1: 10000. Western blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 200. Immunflourescence: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to one month. For long term storage, aliquot and store at -20 °C.

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
 image for anti-PKC alpha antibody (Protein Kinase C, alpha) (ABIN272065) anti-Protein Kinase C, alpha (PKCa) antibody
 image for anti-PKC alpha antibody (Protein Kinase C, alpha) (ABIN272065) anti-Protein Kinase C, alpha (PKCa) antibody (Image 2)