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anti-Rat (Rattus) PKC alpha antibody for ELISA

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C, alpha
  • PRKCA
  • prkca
  • LOC100219195
  • Prkca
Epitope
pThr638
45
25
17
16
16
15
11
10
7
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
256
184
146
29
25
23
17
17
16
5
4
3
3
3
2
1
1
1
Host
Rabbit
221
43
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
8
7
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
210
116
93
59
46
37
27
26
21
19
5
4
4
3
2
1
1
1
1
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Catalog No. ABIN1531462
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN153146 ICC FACS IHC (fro) IF IHC (p) IP IHC RIA ELISA WB Mouse IgG2a Log in to see MC5 13
1 ABIN1531505 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal
1 ABIN3186466 ELISA IHC WB Rabbit IgG Thr287 Log in to see Polyclonal
1 ABIN3182587 ELISA IHC WB Rabbit IgG pThr638 Log in to see Polyclonal
1 ABIN3182236 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal
1 ABIN1532520 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1532477 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1533205 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1585060 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2888746 IHC (p) ELISA WB Rabbit IgG AA 606-655 Log in to see Polyclonal
1 ABIN2888770 ELISA WB Rabbit IgG AA 623-672 Log in to see Polyclonal
1 ABIN3186465 ELISA WB Rabbit IgG Thr286 Log in to see Polyclonal
1 ABIN3186467 ELISA IHC Rabbit IgG Thr288 Log in to see Polyclonal
1 ABIN1456845 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN220831 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 657-672 Log in to see Polyclonal
1 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Log in to see MC5
1 ABIN220827 IP ELISA WB Rabbit IgG Thr641, pThr638 Log in to see Polyclonal
1 ABIN2884227 ELISA IHC (p) WB Rabbit IgG pThr638 Log in to see Polyclonal
1 ABIN2884228 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal
1 ABIN2885163 ELISA WB Rabbit IgG AA 604-653 Log in to see Polyclonal

Top referenced anti-PKC alpha antibody for ELISA

Similar anti-PKC alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 52 Antibodies 70 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 18 Antibodies 32 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 109 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope pThr638
(45), (25), (17), (16), (16), (15), (11), (10), (7), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(256), (184), (146), (29), (25), (23), (17), (17), (16), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(221), (43), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (8), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(210), (116), (93), (59), (46), (37), (27), (26), (21), (19), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) Images
Specificity PKC alpha (Phospho-Thr638) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr638.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638.
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Antigen
Alternative Name PKC alpha (PKCa Antibody Abstract)
Background Molecular weight of target protein: 76 kDa
Synonyms: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C, alpha type
Molecular Weight 76 kDa
Gene ID 5578
OMIM 176960
UniProt P17252
Research Area Cardiovascular, Hypertrophy, Signaling, Protein Modifications, Kinases/Phosphatases
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.531704, Hs.708867 (NCBI Gene Symbol: PRKCA)

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha Antibody (ABIN1531462) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-PKC alpha Antibody (ABIN1531462)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, 2015

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha Antibody (ABIN1531462) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (pThr638) (ABIN1531462) Western blot analysis of extracts from NIH-3T3 cells treated with UV 15', using PKC a...
Immunohistochemistry (IHC) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (pThr638) (ABIN1531462) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC ...