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anti-Rat (Rattus) PKC alpha antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • AAG6
  • AI875142
  • im:7139045
  • LOC100219195
  • pkc-alpha
  • PKC-alpha
  • Pkca
  • PKCA
  • pkcalpha
  • PKRCA
  • PRKACA
  • si:dkey-8l19.1
Epitope
C-Term
49
34
17
15
15
13
12
11
7
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
253
138
114
23
19
19
16
16
16
3
3
2
1
1
Host
Rabbit
222
43
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
10
7
7
5
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
206
123
109
53
49
38
36
22
21
20
15
5
4
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1108731
$ 313.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN272065 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN272062 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN189677 ICC IF IHC (p) IP IHC WB Rabbit IgG Thr638 Log in to see E195
0.0008982679 ABIN189675 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y143
0.0008982679 ABIN220831 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN711568 IHC (p) WB Biotin Rabbit IgG pSer657, pTyr658 Log in to see Polyclonal
0.0008982679 ABIN711575 IHC (p) WB HRP Rabbit IgG pSer657, pTyr658 Log in to see Polyclonal
0.0008982679 ABIN711583 IHC (p) WB Biotin Rabbit IgG pThr637 Log in to see Polyclonal
0.0008982679 ABIN711590 IHC (p) WB HRP Rabbit IgG pThr637 Log in to see Polyclonal
0.0008982679 ABIN408073 ICC IF IHC (p) IHC WB Rabbit IgG Thr495 Log in to see Polyclonal
0.0008982679 ABIN711566 IF (p) IHC (p) WB Rabbit IgG pSer657, pTyr658 Log in to see Polyclonal
0.0008982679 ABIN711581 IF (p) IHC (p) WB Rabbit IgG pThr637 Log in to see Polyclonal
0.0008982679 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345806 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN4345811 ELISA FACS IHC IHC (fro) IHC (p) IP RIA FITC Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345813 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA DyLight 405 Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345814 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 350 Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345815 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB PerCP Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345816 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Allophycocyanin Mouse IgG2a Log in to see MC5
0.0008982679 ABIN4345817 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB DyLight 755 Mouse IgG2a Log in to see MC5

Similar anti-PKC alpha Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 201 Antibodies 101 Antibodies 88 Antibodies
Radioimmunoassay (RIA) 15 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 5 Antibodies 4 Antibodies 1 Antibodies
Immunoprecipitation (IP) 39 Antibodies 26 Antibodies 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 55 Antibodies 39 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 109 Antibodies 59 Antibodies 55 Antibodies

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope C-Term
(49), (34), (17), (15), (15), (13), (12), (11), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(253), (138), (114), (23), (19), (19), (16), (16), (16), (3), (3), (2), (1), (1)
Host Rabbit
(222), (43), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (10), (7), (7), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(206), (123), (109), (53), (49), (38), (36), (22), (21), (20), (15), (5), (4), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity This antibody detects PRKCA (C-term).
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat.
Purification Immunogen affinity purified
Immunogen Synthetic peptide corresponding to the C-terminal of human PKCa .Remarks: Sequence is identical to the related rat and mouse sequence.
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII andγ) and novel (n) PKC isoforms (δ,ε,ζ,ηandθ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC and are independent of Ca2+ On the other hand, nPKC and , as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Reconstitution 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (C-Term) (ABIN1108731) Staining with PRKCA antibody
Western Blotting (WB) image for anti-PKC alpha antibody (Protein Kinase C, alpha) (C-Term) (ABIN1108731) Western blot analysis of Hela cell lysis with PKCalpha antibody