You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Chicken PKC beta antibody for Immunofluorescence

Recommended PKC beta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, beta (PRKCB) Antibodies
  • A130082F03Rik
  • PKC
  • PKC-B
  • PKC-Beta
  • PKC-beta
  • PKCB
  • Pkcb
  • Prkcb
  • Prkcb1
  • PRKCB1
  • prkcb1
  • PRKCB2
  • Prkcb2
  • zgc:63591
Epitope
AA 126-324
44
25
17
12
11
10
9
7
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
139
77
73
12
8
6
5
1
1
1
1
Host
Mouse
157
13
7
6
Clonality
Monoclonal
Conjugate
This PKC beta antibody is un-conjugated
12
9
5
5
5
5
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
156
97
63
23
18
10
7
5
4
3
3
3
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967768
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN967769 IF IP IHC WB Mouse IgG2b AA 126-324 Log in to see 36-PKCb 5

Top referenced anti-PKC beta antibody for Immunofluorescence

Similar anti-PKC beta Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 30 Antibodies 31 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 2 Antibodies
Immunofluorescence (IF) 18 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 63 Antibodies

General

Antigen Protein Kinase C, beta (PRKCB) Antibodies
Epitope AA 126-324
(44), (25), (17), (12), (11), (10), (9), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(139), (77), (73), (12), (8), (6), (5), (1), (1), (1), (1)
Host Mouse
(157), (13), (7), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC beta antibody is un-conjugated
(12), (9), (5), (5), (5), (5), (2)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(156), (97), (63), (23), (18), (10), (7), (5), (4), (3), (3), (3), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-PKC beta Antibody

Target Details PKC beta Application Details Handling References for anti-PKC beta Antibody (ABIN967768) Images
Cross-Reactivity Rat (Rattus), Mouse (Murine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCbeta aa. 126-324
Clone 36-PKCb
Isotype IgG2b

Target Details PKC beta

Product Details anti-PKC beta Antibody Application Details Handling References for anti-PKC beta Antibody (ABIN967768) Images back to top
Antigen
Alternative Name PKC beta (PRKCB Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCbeta is highly expressed in brain and hematopoietic cells. Autophosphorylation of PKCbeta occurs at the N- and C-terminal regions, as well as within the hinge region. However, only the COOH- terminal autophosphorylation sites are essential for PKCbeta's function and subcellular localization. PKCbeta is critical for the proliferation of K562 cells, as well as being an important regulator of human melanogenesis.
Molecular Weight 80kDa
Pathways WNT Signaling

Application Details

Product Details anti-PKC beta Antibody Target Details PKC beta Handling References for anti-PKC beta Antibody (ABIN967768) Images back to top
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details References for anti-PKC beta Antibody (ABIN967768) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-PKC beta Antibody (ABIN967768)

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling Images back to top
Product cited in:

Masur, Lang, Niggemann, Zanker, Entschladen: "High PKC alpha and low E-cadherin expression contribute to high migratory activity of colon carcinoma cells." in: Molecular biology of the cell, Vol. 12, Issue 7, pp. 1973-82, 2001

Stebbins, Mochly-Rosen: "Binding specificity for RACK1 resides in the V5 region of beta II protein kinase C." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 29644-50, 2001

Bell, Burns: "Lipid activation of protein kinase C." in: The Journal of biological chemistry, Vol. 266, Issue 8, pp. 4661-4, 1991

Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988

Images

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling References for anti-PKC beta Antibody (ABIN967768) back to top
Supplier Images
Western Blotting (WB) image for anti-PKC beta antibody (Protein Kinase C, beta) (AA 126-324) (ABIN967768) Western blot analysis of PKCß on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane...