anti-Human PKC beta antibody for Enzyme Immunoassay

Recommended PKC beta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, beta (PRKCB) Antibodies
  • PKC-B
  • Prkcb
  • prkcb1
  • zgc:63591
  • PKC-beta
  • PKCB
  • PRKCB1
  • PRKCB2
  • PKC
  • A130082F03Rik
  • PKC-Beta
  • Pkcb
  • Prkcb1
  • Prkcb2
  • protein kinase C, beta b
  • protein kinase C, beta
  • prkcbb
  • PRKCB
  • Prkcb
Epitope
C-Term
32
17
17
13
13
10
8
7
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
152
109
108
21
20
15
10
6
5
4
2
2
2
2
2
2
1
1
Host
Rabbit
164
16
7
6
Clonality
Polyclonal
Conjugate
This PKC beta antibody is un-conjugated
12
10
5
5
5
5
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
94
73
25
20
10
7
7
5
4
3
3
3
1
1
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Catalog No. ABIN359059
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN401431 EIA WB Rabbit IgG Internal Region Log in to see Polyclonal 4
1 ABIN5610972 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN5540553 EIA IHC (p) WB Goat C-Term, Internal Region Log in to see Polyclonal

General

Antigen Protein Kinase C, beta (PRKCB) Antibodies
Epitope C-Term
(32), (17), (17), (13), (13), (10), (8), (7), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(152), (109), (108), (21), (20), (15), (10), (6), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(164), (16), (7), (6)
Clonality
Polyclonal
Conjugate This PKC beta antibody is un-conjugated
(12), (10), (5), (5), (5), (5), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(172), (94), (73), (25), (20), (10), (7), (7), (5), (4), (3), (3), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-PKC beta Antibody

Target Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN359059) Images
Specificity This antibody reacts to PKC beta 1/2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Isotype Ig

Target Details PKC beta

Product Details anti-PKC beta Antibody Application Details Handling References for anti-PKC beta antibody (ABIN359059) Images back to top
Antigen
Alternative Name PRKCB (PRKCB Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.Synonyms: PKC beta, PKC-B, PKC-beta, PKCB, PRKCB1, Protein kinase C beta type
Gene ID 5579, 9606
UniProt P05771
Pathways WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response

Application Details

Product Details anti-PKC beta Antibody Target Details PKC beta Handling References for anti-PKC beta antibody (ABIN359059) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details References for anti-PKC beta antibody (ABIN359059) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC beta antibody (ABIN359059)

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling Images back to top
Background publications

Chen, Purohit, Halilovic, Doxsey, Newton: "Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4829-39, 2004

Hug, Ahmed, Robbins, Lazar: "A chromatin immunoprecipitation screen reveals protein kinase Cbeta as a direct RUNX1 target gene." in: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 825-30, 2004

Wu, Park: "Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1." in: Biochemical and biophysical research communications, Vol. 311, Issue 4, pp. 948-53, 2003

Becker, Hannun: "cPKC-dependent sequestration of membrane-recycling components in a subset of recycling endosomes." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52747-54, 2003

Birikh, Sklan, Shoham, Soreq: "Interaction of "readthrough" acetylcholinesterase with RACK1 and PKCbeta II correlates with intensified fear-induced conflict behavior." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 283-8, 2003

Images

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN359059) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, beta (PRKCB) (C-Term) antibody (ABIN359059) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Protein Kinase C, beta (PRKCB) (C-Term) antibody (ABIN359059) Western blot analysis of PRKCB (arrow) using PKC beta1/2 Antibody