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|Antigen||Protein Kinase C, beta (PRKCB) Antibodies|
|Conjugate||This PKC beta antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PKC beta AntibodyTarget Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN359059) Images
|Specificity||This antibody reacts to PKC beta 1/2.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
Target Details PKC betaProduct Details anti-PKC beta Antibody Application Details Handling References for anti-PKC beta antibody (ABIN359059) Images back to top
|Alternative Name||PRKCB (PRKCB Antibody Abstract)|
|Background||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.Synonyms: PKC beta, PKC-B, PKC-beta, PKCB, PRKCB1, Protein kinase C beta type|
|Gene ID||5579, 9606|
|Pathways||WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response|
Application DetailsProduct Details anti-PKC beta Antibody Target Details PKC beta Handling References for anti-PKC beta antibody (ABIN359059) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details References for anti-PKC beta antibody (ABIN359059) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PKC beta antibody (ABIN359059)Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling Images back to top
Chen, Purohit, Halilovic, Doxsey, Newton: "Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4829-39, 2004
Hug, Ahmed, Robbins, Lazar: "A chromatin immunoprecipitation screen reveals protein kinase Cbeta as a direct RUNX1 target gene." in: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 825-30, 2004
Wu, Park: "Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1." in: Biochemical and biophysical research communications, Vol. 311, Issue 4, pp. 948-53, 2003
Becker, Hannun: "cPKC-dependent sequestration of membrane-recycling components in a subset of recycling endosomes." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52747-54, 2003
Birikh, Sklan, Shoham, Soreq: "Interaction of "readthrough" acetylcholinesterase with RACK1 and PKCbeta II correlates with intensified fear-induced conflict behavior." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 283-8, 2003
ImagesProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN359059) back to top