You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PKC gamma antibody for Enzyme Immunoassay

Recommended PKC gamma Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, gamma (PRKCG) Antibodies
  • PKC
  • PKC-gamma
  • PKCC
  • Pkcc
  • PKCG
  • PKCgamma
  • PKCI
  • Prkc
  • Prkcc
  • PRKCG
  • RATPKCI
  • SCA14
Epitope
C-Term
38
21
15
14
13
13
8
7
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
138
101
95
3
3
2
1
1
1
1
Host
Rabbit
168
5
1
Clonality
Polyclonal
Conjugate
This PKC gamma antibody is un-conjugated
11
9
7
4
4
4
3
3
3
3
3
3
3
3
3
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
124
74
34
33
21
10
6
4
4
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN359066
$ 390.50
Plus shipping costs $45.00

Top referenced anti-PKC gamma antibody for Enzyme Immunoassay

Similar anti-PKC gamma Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 36 Antibodies 49 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 2 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 66 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Protein Kinase C, gamma (PRKCG) Antibodies
Epitope C-Term
(38), (21), (15), (14), (13), (13), (8), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(138), (101), (95), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(168), (5), (1)
Clonality
Polyclonal
Conjugate This PKC gamma antibody is un-conjugated
(11), (9), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(124), (74), (34), (33), (21), (10), (6), (4), (4), (2), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-PKC gamma Antibody

Target Details PKC gamma Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) Images
Specificity This antibody reacts to PKC gamma.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
Isotype Ig

Target Details PKC gamma

Product Details anti-PKC gamma Antibody Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) Images back to top
Antigen
Alternative Name PRKCG (PRKCG Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).Synonyms: PKC gamma, PKC-gamma, PKCC, PKCG, Protein kinase C gamma type
Gene ID 5582, 9606
UniProt P05129
Pathways WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PKC gamma Antibody Target Details PKC gamma Handling References for anti-PKC gamma Antibody (ABIN359066) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC gamma Antibody Target Details PKC gamma Application Details References for anti-PKC gamma Antibody (ABIN359066) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC gamma Antibody (ABIN359066)

Product Details anti-PKC gamma Antibody Target Details PKC gamma Application Details Handling Images back to top
Background publications

Uchino, Sakai, Kashiwagi, Shirai, Shinohara, Hirose, Iino, Yamamura, Saito: "Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 2254-61, 2004

van de Warrenburg, Verbeek, Piersma, Hennekam, Pearson, Knoers, Kremer, Sinke: "Identification of a novel SCA14 mutation in a Dutch autosomal dominant cerebellar ataxia family." in: Neurology, Vol. 61, Issue 12, pp. 1760-5, 2003

Yabe, Sasaki, Chen, Raskind, Bird, Yamashita, Tsuji, Kikuchi, Tashiro: "Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma." in: Archives of neurology, Vol. 60, Issue 12, pp. 1749-51, 2003

Ananthanarayanan, Stahelin, Digman, Cho: "Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46886-94, 2003

Correia, Duarte, Faro, Pires, Carvalho: "Protein kinase C gamma associates directly with the GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor subunit. Effect on receptor phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6307-13, 2003

Images

Product Details anti-PKC gamma Antibody Target Details PKC gamma Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PKC gamma antibody (Protein Kinase C, gamma) (C-Term) (ABIN359066) Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibo...
Western Blotting (WB) image for anti-PKC gamma antibody (Protein Kinase C, gamma) (C-Term) (ABIN359066) Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma (...