anti-Chemical Rhodopsin antibody for Enzyme Immunoassay

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CG5279
  • DMELRH5
  • Dm Rh5
  • Dmel\\CG5279
  • RH5
  • Rh
  • rh5
  • CG9668
  • DMELRH4
  • Dm Rh4
  • Dmel\\CG9668
  • FBgn0003250
  • RH4
  • rh4
  • CG5192
  • DMELRH6
  • Dm Rh6
  • Dmel\\CG5192
  • R8
  • RH6
  • rh6
  • 143283_at
  • 1F9
  • BEST:GH11778
  • CG4550
  • DMELRH1
  • DRh1
  • Dm Rh1
  • DmRh1
  • Dmel\\CG4550
  • FBgn0002940
  • Nina E
  • NinaE
  • R
  • RH1
  • Rh-1
  • Rh1
  • Rh1(ninaE)
  • Rh1/ninaE
  • opsin
  • ora
  • rh1
  • rh1/ninaE
  • rhodopsin
  • CG10888
  • DMELRH3
  • Dm Rh3
  • Dmel\\CG10888
  • FBgn0003249
  • RH3
  • rh3
  • opn2
  • rp4
  • xrho
  • RHO
  • kfh-rh
  • RDP1
  • CSNBAD1
  • OPN2
  • RP4
  • Noerg1
  • Opn2
  • Ops
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • PIGRHO1
  • ops
  • Rhodopsin 5
  • Rhodopsin 4
  • Rhodopsin 6
  • neither inactivation nor afterpotential E
  • Rhodopsin 3
  • rhodopsin
  • KFH-Rh protein
  • Rh5
  • Rh4
  • Rh6
  • ninaE
  • Rh3
  • RHO
  • rho
  • Rho
Epitope
C-Term
30
18
11
8
5
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Chemical
229
31
17
16
15
3
1
1
1
1
1
1
1
1
Host
Mouse
203
64
5
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
14
12
11
10
8
7
7
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
255
162
162
156
136
93
56
1
1
1
1
Supplier
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Catalog No. ABIN5550991
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5550990 EIA IF IHC (fro) IP WB Mouse IgG1 N-Term Log in to see 4D2

General

Antigen Rhodopsin (RHO) Antibodies
Epitope C-Term
(30), (18), (11), (8), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chemical
(229), (31), (17), (16), (15), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(203), (64), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(14), (12), (11), (10), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(255), (162), (162), (156), (136), (93), (56), (1), (1), (1), (1)
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling Images
Specificity Recognizes Rhodopsin (~40? kDa). Reacts with both native and recombinant protein. No known reactivity to other proteins. The antibody specifically binds to the C-terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of Rhodopsin. Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.
Cross-Reactivity (Details) Species reactivity (tested):Vertebrates.
Characteristics Synonyms: RHO, Opsin-2, OPN2
Purification Protein G Chromatography
Immunogen Bovine Rhodopsin.
Clone 1D4
Isotype IgG1

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Target Type Chemical
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (Ref.2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO
Molecular Weight 48 kDa
Gene ID 6010
UniProt P08100
Research Area Neurology
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top
Application Notes ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS pH 7.4, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months