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|Application / Reactivity||Chemical|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||99 Antibodies|
|Immunofluorescence (IF)||108 Antibodies|
|Immunohistochemistry (IHC)||89 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||32 Antibodies|
|Immunoprecipitation (IP)||60 Antibodies|
|Western Blotting (WB)||141 Antibodies|
|Antigen||Rhodopsin (RHO) Antibodies|
|Conjugate||This Rhodopsin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Rhodopsin AntibodyTarget Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN180439) Images
This antibody detects Rhodopsin.
Cellular Localization: Cytoplasmic
|Characteristics||Synonyms: RHO, Opsin-2, OPN2|
|Purification||Protein G chromatography|
|Immunogen||Membrane preparation from adult Rat Retina. Remarks: Mol. Wt. of Antigen: 39kDa|
Target Details RhodopsinProduct Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin Antibody (ABIN180439) Images back to top
|Alternative Name||Rhodopsin (RHO Antibody Abstract)|
|Background||Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.Synonyms: OPN2, Opsin-2, RHO|
Application DetailsProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin Antibody (ABIN180439) Images back to top
Immunohistochemistry on formalin/paraffin embedded sections: Ab 1-2 μg/mL for 60 minat RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citratebuffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Western Blotting: Ab 1-2 micrograms/mL for 2hrs at RT. Immunofluorescence. Recommended positive control: Brain or retina.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin Antibody (ABIN180439) Images back to top
|Buffer||10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Rhodopsin Antibody (ABIN180439)Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Treisman, Morabito, Barnstable: "Opsin expression in the rat retina is developmentally regulated by transcriptional activation." in: Molecular and cellular biology, Vol. 8, Issue 4, pp. 1570-9, 1988
Hicks, Barnstable: "Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 35, Issue 11, pp. 1317-28, 1987
Fekete, Barnstable: "The subcellular localization of rat photoreceptor-specific antigens." in: Journal of neurocytology, Vol. 12, Issue 5, pp. 785-803, 1984
Barnstable: "Monoclonal antibodies which recognize different cell types in the rat retina." in: Nature, Vol. 286, Issue 5770, pp. 231-5, 1980
ImagesProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN180439) back to top