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anti-Cow (Bovine) Rhodopsin antibody for Immunohistochemistry

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • 1F9
  • 143283_at
  • BEST:GH11778
  • CG4550
  • CG5192
  • CG5279
  • CG9668
  • CG10888
  • CSNBAD1
  • DMELRH1
  • DMELRH3
  • DMELRH4
  • DMELRH5
  • DMELRH6
  • Dmel\\CG4550
  • Dmel\\CG5192
  • Dmel\\CG5279
  • Dmel\\CG9668
  • Dmel\\CG10888
  • Dm Rh1
  • DmRh1
  • Dm Rh3
  • Dm Rh4
  • Dm Rh5
  • Dm Rh6
  • DRh1
  • FBgn0002940
  • FBgn0003249
  • FBgn0003250
  • fi06d11
  • kfh-rh
  • NinaE
  • Nina E
  • Noerg1
  • opn2
  • Opn2
  • OPN2
  • ops
  • Ops
  • opsin
  • ora
  • PIGRHO1
  • R
  • R8
  • RDP1
  • Rh
  • Rh-1
  • RH1
  • rh1
  • Rh1
  • Rh1(ninaE)
  • Rh1/ninaE
  • rh1/ninaE
  • RH3
  • rh3
  • RH4
  • rh4
  • RH5
  • rh5
  • RH6
  • rh6
  • RHO
  • rhodopsin
  • RP4
  • rp4
  • wu:fi06d11
  • xrho
  • zfo2
  • zfrho
Reactivity
Human, Cow (Bovine), Mouse (Murine), Pig (Porcine), Rat (Rattus)
85
37
22
12
4
2
2
1
1
1
Host
Mouse
113
35
3
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
8
6
6
6
6
4
4
4
4
4
4
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
141
110
90
80
74
68
19
4
4
1
1
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Catalog No. ABIN1580458
$ 220.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001136324 ABIN956749 IHC WB Mouse IgG1 Log in to see 1D4
0.001136324 ABIN1103993 ICC IP IHC ELISA WB Mouse IgG1 Log in to see Rho 4D2
0.001136324 ABIN1952414 ICC IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952432 ICC IP IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952462 ICC IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952420 ICC IP IHC ELISA WB APC Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952426 ICC IP IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952456 ICC IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952438 ICC IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952444 ICC IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952527 ICC IP IHC ELISA WB PE Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952450 ICC IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952468 ICC IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952533 ICC IP IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952492 ICC IP IHC ELISA WB FITC Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952474 ICC IP IHC ELISA WB Biotin Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952515 ICC IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952521 ICC IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 4D2
0.001136324 ABIN1952411 ICC IF IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 1D4
0.001136324 ABIN1952441 ICC IF IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 1D4

Similar anti-Rhodopsin Antibodies

Application / Reactivity Rat (Rattus) Cow (Bovine) Human Mouse (Murine) Pig (Porcine)
Western Blotting (WB) 13 Antibodies 38 Antibodies 22 Antibodies 5 Antibodies 3 Antibodies
Immunoprecipitation (IP) 33 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 6 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 11 Antibodies 6 Antibodies 37 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Human, Cow (Bovine), Mouse (Murine), Pig (Porcine), Rat (Rattus)
(85), (37), (22), (12), (4), (2), (2), (1), (1), (1)
Host Mouse
(113), (35), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(8), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(141), (110), (90), (80), (74), (68), (19), (4), (4), (1), (1)
Pubmed 2 references available
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN1580458) Images
Purification affinity purified antibody
Clone B630
Isotype IgG1

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin Antibody (ABIN1580458) Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Background Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha, and a beta,gamma, subunit. The alpha, subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-B630 was raised against bovine outer segment discs and shows convincing staining for rhodopsin both on western blots and on sections of retina. The protein is concentrated in rod outer segments. The HGNC name for this protein is RHO.
Research Area Neurology
Pathways WNT Signaling

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin Antibody (ABIN1580458) Images back to top
Application Notes Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin Antibody (ABIN1580458) Images back to top
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 4°C short term or -20°C long term.

References for anti-Rhodopsin Antibody (ABIN1580458)

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Background publications

Molday: "Photoreceptor membrane proteins, phototransduction, and retinal degenerative diseases. The Friedenwald Lecture." in: Investigative ophthalmology & visual science, Vol. 39, Issue 13, pp. 2491-513, 1998

Yau: "Phototransduction mechanism in retinal rods and cones. The Friedenwald Lecture." in: Investigative ophthalmology & visual science, Vol. 35, Issue 1, pp. 9-32, 1994

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN1580458) back to top
Supplier Images
Western Blotting (WB) image for anti-Rhodopsin antibody (RHO) (ABIN1580458) Blot of bovine retinal extracts probed with ABIN1580458. The antibody stains a band c...
Immunofluorescence (IF) image for anti-Rhodopsin antibody (RHO) (ABIN1580458) High magnification confocal image of pig retinal section stained with ABIN1580458 (Gr...