You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Rhodopsin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • 1F9
  • 143283_at
  • BEST:GH11778
  • CG4550
  • CG5192
  • CG5279
  • CG9668
  • CG10888
  • CSNBAD1
  • DMELRH1
  • DMELRH3
  • DMELRH4
  • DMELRH5
  • DMELRH6
  • Dmel\\CG4550
  • Dmel\\CG5192
  • Dmel\\CG5279
  • Dmel\\CG9668
  • Dmel\\CG10888
  • Dm Rh1
  • DmRh1
  • Dm Rh3
  • Dm Rh4
  • Dm Rh5
  • Dm Rh6
  • DRh1
  • FBgn0002940
  • FBgn0003249
  • FBgn0003250
  • fi06d11
  • kfh-rh
  • NinaE
  • Nina E
  • Noerg1
  • opn2
  • Opn2
  • OPN2
  • ops
  • Ops
  • opsin
  • ora
  • PIGRHO1
  • R
  • R8
  • RDP1
  • Rh
  • Rh-1
  • RH1
  • rh1
  • Rh1
  • Rh1(ninaE)
  • Rh1/ninaE
  • rh1/ninaE
  • RH3
  • rh3
  • RH4
  • rh4
  • RH5
  • rh5
  • RH6
  • rh6
  • RHO
  • rhodopsin
  • RP4
  • rp4
  • wu:fi06d11
  • xrho
  • zfo2
  • zfrho
Epitope
2nd Extracellular Domain
27
17
8
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
152
38
22
13
5
3
2
1
1
1
Host
Rabbit
181
34
3
1
Clonality
Polyclonal
Conjugate
This Rhodopsin antibody is un-conjugated
10
10
8
8
6
6
6
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
209
142
138
136
117
107
45
4
4
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1049292
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN2888702 IHC (p) ELISA WB Rabbit IgG AA 299-348 Log in to see Polyclonal
0.0008982679 ABIN1960118 IF IHC (p) IHC WB Mouse IgG1, kappa Log in to see A531
0.0008982679 ABIN1960117 ICC IF IHC (p) WB Mouse IgG1, kappa Log in to see B630
0.0008982679 ABIN408082 ICC IF IHC (p) IHC WB Rabbit IgG Leu328 Log in to see Polyclonal
0.0008982679 ABIN447325 IHC (fro) IHC (p) IHC WB Sheep Log in to see Polyclonal

Similar anti-Rhodopsin Antibodies

Application / Reactivity Human
ELISA 11 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 22 Antibodies

General

Antigen Rhodopsin (RHO) Antibodies
Epitope 2nd Extracellular Domain
(27), (17), (8), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(152), (38), (22), (13), (5), (3), (2), (1), (1), (1)
Host Rabbit
(181), (34), (3), (1)
Clonality
Polyclonal
Conjugate This Rhodopsin antibody is un-conjugated
(10), (10), (8), (8), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(209), (142), (138), (136), (117), (107), (45), (4), (4), (1), (1)
Supplier Log in to see

Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling Images
Specificity Human RHO / Rhodopsin. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Cross-Reactivity Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).
Purification Immunoaffinity Chromatography
Immunogen Synthetic 19 amino acid peptide from 2nd extracellular domain of human RHO / Rhodopsin. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).
ImmunogenType: Synthetic peptide

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rhodopsin / rho (RHO Antibody Abstract)
Target Type Chemical
Background Standard Gene Symbol: RHO, Gene Family: GPCR, Gene Subfamily: Opsin, Synonyms: RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2
Gene ID 6010
Pathways WNT Signaling

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top
Application Notes IHC-P (1 - 3 μg/mL),
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-80 °C
Storage Comment Long term: -70°C, Short term: +4°C

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Rhodopsin antibody (RHO) (2nd Extracellular Domain) (ABIN1049292) Anti-RHO / Rhodopsin antibody ABIN1049292 IHC staining of human eye, retina. Immunoh...