|Antigen||Transcription Factor 4 (TCF4) Antibodies|
|Epitope||AA 31-331 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TCF4 antibody is un-conjugated Alternatives|
Electrophoretic Mobility-Shift Assay (EMSA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-TCF4 AntibodyTarget Details TCF4 Application Details Handling References for anti-TCF4 antibody (ABIN264394) Images
|Characteristics||Synonyms: T-Cell Factor-3/4, TCF-3, TCF-4|
|Immunogen||His-tagged fusion protein corresponding to amino acids 31-331 of the human TCF.|
Target Details TCF4Product Details anti-TCF4 Antibody Application Details Handling References for anti-TCF4 antibody (ABIN264394) Images back to top
|Alternative Name||TCF4 (TCF4 Antibody Abstract)|
|Background||TCF-3 and TCF-4 (T cell factor-4) are a members of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia. TCF-3 appears to be expressed exclusively of TCF-4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.Synonyms: E2A, ITF-1, ITF-1, ITF-2, ITF1, ITF1, ITF2, SEF-2, SEF2, TCF-3, TCF-3, TCF-4, TCF-4, Transcription factor 3, Transcription factor E2-alpha|
|Research Area||Stem Cells, Wnt, Transcription Factors, Wnt Signaling|
|Pathways||WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus|
Application DetailsProduct Details anti-TCF4 Antibody Target Details TCF4 Handling References for anti-TCF4 antibody (ABIN264394) Images back to top
Western Blotting: Detects TCF-4 at 1-4 μg/mL and a 60 kDa band in Cos-1 cells transfectedwith human TCF-3. Immunohistochemistry (Paraffin Section Staining). Gel Shift.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TCF4 Antibody Target Details TCF4 Application Details References for anti-TCF4 antibody (ABIN264394) Images back to top
|Buffer||0.1 M Tris-Glycine, pH 7.4, 150 mM Sodium Chloride, 30 % Glycerol and 0.08 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-TCF4 antibody (ABIN264394)Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling Images back to top
|Product cited in:||
Denys, Jadidizadeh, Amini Nik, Van Dam, Aerts, Alman, Cassiman, Tejpar: "Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors." in: Oncogene, Vol. 23, Issue 3, pp. 654-64, 2004
Barker, Huls, Korinek, Clevers: "Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium." in: The American journal of pathology, Vol. 154, Issue 1, pp. 29-35, 1999
ImagesProduct Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling References for anti-TCF4 antibody (ABIN264394) back to top