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anti-Mouse (Murine) TCF4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TCF4 Antibody (supplied by: Log in to see )

Antigen
Transcription Factor 4 (TCF4) Antibodies
  • 5730422P05Rik
  • ASP-I2
  • bHLHb19
  • E2-2
  • e2-A
  • E2.2
  • GE2
  • ITF-2
  • ITF-2b
  • ITF2
  • ME2
  • MITF-2A
  • MITF-2B
  • mTcf-4B
  • mTcf-4E
  • PTHS
  • SEF-2
  • SEF2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • Tcf-4
  • TCF-4
  • tcf-4
  • TCF4
  • Tcf4
  • TCF4B
  • TCF4E
  • TCF7L2
  • TFE
  • XE2
  • Xtcf-4
  • Xtcf4
Epitope
AA 31-331
24
15
5
4
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
82
18
12
4
2
1
1
1
1
Host
Mouse
59
14
9
Clonality
Monoclonal
Conjugate
This TCF4 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
74
41
21
18
12
11
7
4
2
1
1
1
Supplier
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Catalog No. ABIN264393
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089850515 ABIN264394 EMSA IHC (p) WB Mouse IgG1 AA 31-331 Log in to see 6F12-3 2
0.00089850515 ABIN187661 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see 6H5-3

Top referenced anti-TCF4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-TCF4 Antibodies

Application / Reactivity Mouse (Murine) Human
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies 1 Antibodies
ELISA 6 Antibodies 41 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 19 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Flow Cytometry (FACS) 18 Antibodies

General

Antigen Transcription Factor 4 (TCF4) Antibodies
Epitope AA 31-331
(24), (15), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(82), (18), (12), (4), (2), (1), (1), (1), (1)
Host Mouse
(59), (14), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TCF4 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(74), (41), (21), (18), (12), (11), (7), (4), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-TCF4 Antibody

Target Details TCF4 Application Details Handling References for anti-TCF4 Antibody (ABIN264393) Images
Characteristics Synonyms: Transcription factor 4, TCF-4, ITF2, ITF-2, SEF2, SEF-2, Immunoglobulin transcription factor2, SL3-3 enhancer factor 2
Purification Purified
Immunogen Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein. Genename: TCF7L2
Clone 6H5-3
Isotype IgG2a

Target Details TCF4

Product Details anti-TCF4 Antibody Application Details Handling References for anti-TCF4 Antibody (ABIN264393) Images back to top
Antigen
Alternative Name TCF4 (TCF4 Antibody Abstract)
Background TCF-4 (T Cell Factor 4) is a member of the TCF/LEF (Lymphoid Enhancer Factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.Synonyms: ITF-2, ITF2, Immunoglobulin transcription factor 2, SEF-2, SEF2, SL3-3 enhancer factor 2, TCF-4, Transcription factor 4
Gene ID 6925
UniProt P15884
Research Area Stem Cells, Wnt, Transcription Factors, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-TCF4 Antibody Target Details TCF4 Handling References for anti-TCF4 Antibody (ABIN264393) Images back to top
Application Notes Western Blotting: Detects a 66 kDa band in Cos-1 cells transfected with human TCF-4 (1-4g/mL). Immunoprecipitation. Immunohistochemistry of 4 % paraformaldehyde fixed tissues. Gel Shift.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details References for anti-TCF4 Antibody (ABIN264393) Images back to top
Buffer 0.7 M Tris-Glycine, pH 7.4, 0.107 M Sodium Chloride, 30 % Glycerol, 0.035 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-TCF4 Antibody (ABIN264393)

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling Images back to top
Product cited in:

Shimura, Takenaka, Tsutsumi, Hogan, Kikuchi, Raz: "Galectin-3, a novel binding partner of beta-catenin." in: Cancer research, Vol. 64, Issue 18, pp. 6363-7, 2004

Uematsu, Kanazawa, You, He, Xu, Li, Peterlin, McCormick, Jablons: "Wnt pathway activation in mesothelioma: evidence of Dishevelled overexpression and transcriptional activity of beta-catenin." in: Cancer research, Vol. 63, Issue 15, pp. 4547-51, 2003

Background publications

Barker, Huls, Korinek, Clevers: "Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium." in: The American journal of pathology, Vol. 154, Issue 1, pp. 29-35, 1999

Ishitani, Ninomiya-Tsuji, Nagai, Nishita, Meneghini, Barker, Waterman, Bowerman, Clevers, Shibuya, Matsumoto: "The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF." in: Nature, Vol. 399, Issue 6738, pp. 798-802, 1999

Korinek, Barker, Moerer, van Donselaar, Huls, Peters, Clevers: "Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4." in: Nature genetics, Vol. 19, Issue 4, pp. 379-83, 1998

Images

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling References for anti-TCF4 Antibody (ABIN264393) back to top
Supplier Images
Western Blotting (WB) image for anti-TCF4 antibody (Transcription Factor 4) (AA 31-331) (ABIN264393) anti-Transcription Factor 4 (TCF4) (AA 31-331) antibody