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anti-Human WIF1 antibody for Enzyme Immunoassay

Recommended WIF1 Antibody (supplied by: Log in to see )

Antigen
WNT Inhibitory Factor 1 (WIF1) Antibodies
  • AW107799
  • GB10372
  • shf
  • WIF-1
  • wif-1
  • WIF1
  • zgc:110507
Epitope
C-Term
35
24
15
13
4
3
2
2
2
2
2
1
1
Reactivity
Human
102
20
19
Host
Rabbit
89
16
2
Clonality
Polyclonal
Conjugate
This WIF1 antibody is un-conjugated
7
7
6
6
6
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
86
55
43
14
13
11
5
2
1
1
1
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Catalog No. ABIN357861
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088337954 ABIN357860 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.00088337954 ABIN1109510 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1G5 1

Top referenced anti-WIF1 antibody for Enzyme Immunoassay

Similar anti-WIF1 Antibodies

Application / Reactivity Human
ELISA 53 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Western Blotting (WB) 84 Antibodies

General

Antigen WNT Inhibitory Factor 1 (WIF1) Antibodies
Epitope C-Term
(35), (24), (15), (13), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Reactivity Human
(102), (20), (19)
Host Rabbit
(89), (16), (2)
Clonality
Polyclonal
Conjugate This WIF1 antibody is un-conjugated
(7), (7), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(86), (55), (43), (14), (13), (11), (5), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-WIF1 Antibody

Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN357861) Images
Specificity This antibody detects WIF1 (C-term).
Purification Protein A Chromatography followed by peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human WIF1.
Isotype Ig

Target Details WIF1

Product Details anti-WIF1 Antibody Application Details Handling References for anti-WIF1 Antibody (ABIN357861) Images back to top
Antigen
Alternative Name WIF1 (WIF1 Antibody Abstract)
Background WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.Synonyms: UNQ191/PRO217, WIF-1, Wnt inhibitory factor 1
Molecular Weight 41528 Da
Gene ID 11197, 9606
UniProt Q9Y5W5
Research Area Stem Cells, Wnt, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-WIF1 Antibody Target Details WIF1 Handling References for anti-WIF1 Antibody (ABIN357861) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistchemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details References for anti-WIF1 Antibody (ABIN357861) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-WIF1 Antibody (ABIN357861)

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling Images back to top
Background publications

Elston, Gill, Conaglen, Clarkson, Shaw, Law, Cook, Little, Clifton-Bligh, Robinson, McDonald: "Wnt pathway inhibitors are strongly down-regulated in pituitary tumors." in: Endocrinology, Vol. 149, Issue 3, pp. 1235-42, 2008

Clément, Guilleret, He, Yagui-Beltrán, Lin, You, Xu, Shi, Okamoto, Benhattar, Jablons: "Epigenetic alteration of the Wnt inhibitory factor-1 promoter occurs early in the carcinogenesis of Barrett's esophagus." in: Cancer science, Vol. 99, Issue 1, pp. 46-53, 2007

Chan, Cui, van Hasselt, Li, Srivastava, Jin, Ng, Wang, Lee, Tsao, Zhong, Robertson, Rha, Chan, Tao: "The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 7, pp. 644-50, 2007

Images

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN357861) back to top
Supplier Images
 image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (C-Term) (ABIN357861) anti-WNT Inhibitory Factor 1 (WIF1) (C-Term) antibody
 image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (C-Term) (ABIN357861) anti-WNT Inhibitory Factor 1 (WIF1) (C-Term) antibody (Image 2)