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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||14 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies|
|Western Blotting (WB)||83 Antibodies|
|Antigen||WNT Inhibitory Factor 1 (WIF1) Antibodies|
|Conjugate||This WIF1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-WIF1 AntibodyTarget Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN357861) Images
|Specificity||This antibody detects WIF1 (C-term).|
|Purification||Protein A Chromatography followed by peptide affinity purification.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human WIF1.|
Target Details WIF1Product Details anti-WIF1 Antibody Application Details Handling References for anti-WIF1 Antibody (ABIN357861) Images back to top
|Alternative Name||WIF1 (WIF1 Antibody Abstract)|
|Background||WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.Synonyms: UNQ191/PRO217, WIF-1, Wnt inhibitory factor 1|
|Molecular Weight||41528 Da|
|Gene ID||11197, 9606|
|Research Area||Stem Cells, Wnt, Wnt Signaling|
Application DetailsProduct Details anti-WIF1 Antibody Target Details WIF1 Handling References for anti-WIF1 Antibody (ABIN357861) Images back to top
ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistchemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WIF1 Antibody Target Details WIF1 Application Details References for anti-WIF1 Antibody (ABIN357861) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-WIF1 Antibody (ABIN357861)Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling Images back to top
Elston, Gill, Conaglen et al.: "Wnt pathway inhibitors are strongly down-regulated in pituitary tumors." in: Endocrinology, Vol. 149, Issue 3, pp. 1235-42, 2008 (PubMed).
Clément, Guilleret, He et al.: "Epigenetic alteration of the Wnt inhibitory factor-1 promoter occurs early in the carcinogenesis of Barrett's esophagus." in: Cancer science, Vol. 99, Issue 1, pp. 46-53, 2007 (PubMed).
Chan, Cui, van Hasselt et al.: "The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 7, pp. 644-50, 2007 (PubMed).
ImagesProduct Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN357861) back to top