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anti-Human WIF1 antibody for Western Blotting

Recommended WIF1 Antibody (supplied by: Log in to see )

Antigen
WNT Inhibitory Factor 1 (WIF1) Antibodies
  • AW107799
  • GB10372
  • shf
  • WIF-1
  • wif-1
  • WIF1
  • zgc:110507
Epitope
AA 51-80, N-Term
36
25
15
13
4
3
2
2
2
2
1
1
1
Reactivity
Human
107
20
19
Host
Rabbit
92
18
2
Clonality
Polyclonal
Conjugate
This WIF1 antibody is un-conjugated
7
7
6
6
6
5
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
56
46
16
15
11
6
3
1
1
1
Supplier
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Catalog No. ABIN389184
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13357262 ABIN658480 WB Rabbit Ig AA 344-373, C-Term Log in to see Polyclonal 2
0.13357262 ABIN389185 IHC (p) WB Rabbit Ig AA 347-376, C-Term Log in to see Polyclonal 2
0.13357262 ABIN969457 ICC IHC ELISA WB Mouse IgG1 Log in to see 1G5 2
0.13357262 ABIN2784868 WB Rabbit N-Term Log in to see Polyclonal
0.13357262 ABIN1109510 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1G5 1
0.13357262 ABIN1876574 IHC WB Rabbit IgG Log in to see Polyclonal
0.13357262 ABIN2501200 IF IHC ELISA WB Mouse IgG1 Log in to see 1G5
0.13357262 ABIN2494115 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13357262 ABIN928627 WB Rabbit N-Term Log in to see Polyclonal
0.13357262 ABIN2406130 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.13357262 ABIN524616 WB Mouse AA 1-379, full length Log in to see Polyclonal
0.13357262 ABIN524617 WB Rabbit AA 1-379, full length Log in to see Polyclonal
0.13357262 ABIN2494116 IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.13357262 ABIN2494117 IHC ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13357262 ABIN1845471 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0011713472 ABIN357861 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 3
0.0011713472 ABIN357860 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.0011713472 ABIN751108 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal
0.0011713472 ABIN1501753 ICC IF IHC ELISA WB Mouse IgG1 Log in to see 1G5
0.0011713472 ABIN967258 IF IHC ELISA WB Mouse IgG1 Log in to see 1G5 3

Top referenced anti-WIF1 antibody for Western Blotting

Similar anti-WIF1 Antibodies

Application / Reactivity Human
ELISA 54 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Western Blotting (WB) 88 Antibodies

General

Antigen WNT Inhibitory Factor 1 (WIF1) Antibodies
Epitope AA 51-80, N-Term
(36), (25), (15), (13), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(107), (20), (19)
Host Rabbit
(92), (18), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This WIF1 antibody is un-conjugated
(7), (7), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (56), (46), (16), (15), (11), (6), (3), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-WIF1 Antibody

Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN389184) Images
Specificity This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-term region of human WIF1.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 AA from the N-terminal region of human WIF1.
Clone RB10582
Isotype Ig

Target Details WIF1

Product Details anti-WIF1 Antibody Application Details Handling References for anti-WIF1 Antibody (ABIN389184) Images back to top
Antigen
Alternative Name WIF1 (WIF1 Antibody Abstract)
Background WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Synonyms: Wnt inhibitory factor 1, WIF-1, WIF1
Molecular Weight 41528 DA
Gene ID 11197
UniProt Q9Y5W5
Research Area Stem Cells, Cell Cycle, Cell Structure
Pathways WNT Signaling

Application Details

Product Details anti-WIF1 Antibody Target Details WIF1 Handling References for anti-WIF1 Antibody (ABIN389184) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details References for anti-WIF1 Antibody (ABIN389184) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-WIF1 Antibody (ABIN389184)

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling Images back to top
Product cited in:

Zhou, Chen, Mashrah, Zhu, He, Hu, Xiang, Yao, Guo, Zhang: "Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis." in: Oncology reports, Vol. 34, Issue 5, pp. 2636-42, 2015

Background publications

Elston, Gill, Conaglen, Clarkson, Shaw, Law, Cook, Little, Clifton-Bligh, Robinson, McDonald: "Wnt pathway inhibitors are strongly down-regulated in pituitary tumors." in: Endocrinology, Vol. 149, Issue 3, pp. 1235-42, 2008

Chan, Cui, van Hasselt, Li, Srivastava, Jin, Ng, Wang, Lee, Tsao, Zhong, Robertson, Rha, Chan, Tao: "The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 7, pp. 644-50, 2007

Images

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN389184) back to top
Supplier Images
Western Blotting (WB) image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (AA 51-80) (ABIN389184) Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) ...
Immunohistochemistry (IHC) image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (AA 51-80) (ABIN389184) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (H...