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anti-Human WIF1 antibody for Western Blotting

Recommended WIF1 Antibody (supplied by: Log in to see )

Antigen
WNT Inhibitory Factor 1 (WIF1) Antibodies
  • AW107799
  • GB10372
  • shf
  • WIF-1
  • wif-1
  • WIF1
  • zgc:110507
Epitope
AA 51-80, N-Term
36
24
15
13
4
3
2
2
2
2
1
1
1
Reactivity
Human
107
20
20
Host
Rabbit
91
19
3
Clonality
Polyclonal
Conjugate
This WIF1 antibody is un-conjugated
7
7
6
6
6
5
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
91
57
47
15
15
11
7
3
1
1
1
Supplier
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Catalog No. ABIN389184
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12917736 ABIN658480 WB Rabbit Ig AA 344-373, C-Term Log in to see Polyclonal 2
0.12917736 ABIN389185 IHC (p) WB Rabbit Ig AA 347-376, C-Term Log in to see Polyclonal 2
0.12917736 ABIN969457 ICC IHC ELISA WB Mouse IgG1 Log in to see 1G5 2
0.12917736 ABIN2784868 WB Rabbit N-Term Log in to see Polyclonal
0.12917736 ABIN1109510 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1G5 1
0.12917736 ABIN1876574 IHC WB Rabbit IgG Log in to see Polyclonal
0.12917736 ABIN2501200 IF IHC ELISA WB Mouse IgG1 Log in to see 1G5
0.12917736 ABIN2494115 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12917736 ABIN2406130 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.12917736 ABIN524616 WB Mouse AA 1-379, full length Log in to see Polyclonal
0.12917736 ABIN524617 WB Rabbit AA 1-379, full length Log in to see Polyclonal
0.12917736 ABIN2494117 IHC ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12917736 ABIN2494116 IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.12917736 ABIN928627 WB Rabbit N-Term Log in to see Polyclonal
0.12917736 ABIN1845471 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.12917736 ABIN4219616 WB Rabbit N-Term Log in to see Polyclonal
0.001181995 ABIN4620472 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.001181995 ABIN4620473 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 3
0.001181995 ABIN751108 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal
0.001181995 ABIN967258 IF IHC ELISA WB Mouse IgG1 Log in to see 1G5 3

Top referenced anti-WIF1 antibody for Western Blotting

Similar anti-WIF1 Antibodies

Application / Reactivity Human
ELISA 55 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Western Blotting (WB) 88 Antibodies

General

Antigen WNT Inhibitory Factor 1 (WIF1) Antibodies
Epitope AA 51-80, N-Term
(36), (24), (15), (13), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(107), (20), (20)
Host Rabbit
(91), (19), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This WIF1 antibody is un-conjugated
(7), (7), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(91), (57), (47), (15), (15), (11), (7), (3), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-WIF1 Antibody

Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN389184) Images
Specificity This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-term region of human WIF1.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 AA from the N-terminal region of human WIF1.
Clone RB10582
Isotype Ig

Target Details WIF1

Product Details anti-WIF1 Antibody Application Details Handling References for anti-WIF1 Antibody (ABIN389184) Images back to top
Antigen
Alternative Name WIF1 (WIF1 Antibody Abstract)
Background WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Synonyms: Wnt inhibitory factor 1, WIF-1, WIF1
Molecular Weight 41528 DA
Gene ID 11197
UniProt Q9Y5W5
Research Area Stem Cells, Cell Cycle, Cell Structure
Pathways WNT Signaling

Application Details

Product Details anti-WIF1 Antibody Target Details WIF1 Handling References for anti-WIF1 Antibody (ABIN389184) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details References for anti-WIF1 Antibody (ABIN389184) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-WIF1 Antibody (ABIN389184)

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling Images back to top
Product cited in:

Zhou, Chen, Mashrah, Zhu, He, Hu, Xiang, Yao, Guo, Zhang: "Expression and promoter methylation of Wnt inhibitory factor-1 in the development of oral submucous fibrosis." in: Oncology reports, Vol. 34, Issue 5, pp. 2636-42, 2015

Background publications

Elston, Gill, Conaglen, Clarkson, Shaw, Law, Cook, Little, Clifton-Bligh, Robinson, McDonald: "Wnt pathway inhibitors are strongly down-regulated in pituitary tumors." in: Endocrinology, Vol. 149, Issue 3, pp. 1235-42, 2008

Chan, Cui, van Hasselt, Li, Srivastava, Jin, Ng, Wang, Lee, Tsao, Zhong, Robertson, Rha, Chan, Tao: "The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 7, pp. 644-50, 2007

Images

Product Details anti-WIF1 Antibody Target Details WIF1 Application Details Handling References for anti-WIF1 Antibody (ABIN389184) back to top
Supplier Images
Western Blotting (WB) image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (AA 51-80) (ABIN389184) Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) ...
Immunohistochemistry (IHC) image for anti-WIF1 antibody (WNT Inhibitory Factor 1) (AA 51-80) (ABIN389184) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (H...