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anti-Human WNT1 antibody for Western Blotting

Recommended WNT1 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • wingless-type MMTV integration site family, member 1
  • wingless-related MMTV integration site 1
  • WNT1
  • wnt1
  • Wnt1
  • WNT-1
Epitope
Middle Region
15
14
8
6
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
106
50
22
11
7
7
7
5
5
3
3
2
2
2
1
1
Host
Rabbit
89
17
Clonality
Polyclonal
Conjugate
This WNT1 antibody is un-conjugated
5
1
1
1
1
1
Application
Western Blotting (WB)
85
45
32
29
16
15
7
5
2
2
1
1
1
Options
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Catalog No. ABIN2779465
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870732 ABIN453464 EIA WB Rabbit C-Term Log in to see Polyclonal 1
3.1870732 ABIN390755 FACS IHC (p) WB Rabbit Ig AA 260-288, C-Term Log in to see Polyclonal 1
3.1870732 ABIN733748 IF (p) IHC (p) WB Rabbit IgG AA 255-300 Log in to see Polyclonal 3
3.1870732 ABIN3042350 WB Rabbit IgG AA 45-74, N-Term Log in to see Polyclonal
3.1870732 ABIN969458 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 10C8 1
3.1870732 ABIN964686 WB Mouse IgG1 kappa Internal Region Log in to see 13F9
3.1870732 ABIN2856226 WB Rabbit IgG Center Log in to see Polyclonal
3.1870732 ABIN964685 WB Mouse IgG1 kappa Internal Region Log in to see 13F9
3.1870732 ABIN233837 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1870732 ABIN223266 WB Rabbit IgG Log in to see Polyclonal
3.1870732 ABIN1687685 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.1870732 ABIN3187496 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.1870732 ABIN2779466 WB Rabbit C-Term Log in to see Polyclonal
3.1870732 ABIN2705330 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.1870732 ABIN408121 ICC IF IHC IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
3.1870732 ABIN4905627 WB Rabbit IgG Log in to see Polyclonal
3.1870732 ABIN635697 WB Rabbit Middle Region Log in to see Polyclonal
3.1870732 ABIN3029624 FACS IHC ELISA WB Rabbit Ig Fraction AA 260-288 Log in to see Polyclonal
3.1870732 ABIN1109511 EIA IF IHC (p) WB Mouse IgG1 Log in to see 10C8
3.1870732 ABIN1846734 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see

Top referenced anti-WNT1 antibody for Western Blotting

Similar anti-WNT1 Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Rat (Rattus) Dog (Canine) Guinea Pig Horse (Equine) Rabbit Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 86 Antibodies 39 Antibodies 19 Antibodies 10 Antibodies 7 Antibodies 4 Antibodies 4 Antibodies 4 Antibodies 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies 18 Antibodies 8 Antibodies 3 Antibodies 2 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
Epitope Middle Region
(15), (14), (8), (6), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(106), (50), (22), (11), (7), (7), (7), (5), (5), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(89), (17)
Clonality
Polyclonal
Conjugate This WNT1 antibody is un-conjugated
(5), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(85), (45), (32), (29), (16), (15), (7), (5), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT1 Antibody

Target Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) Images
Blocking Peptide Blocking peptide for this product available: ABIN986208
Sequence FGREFVDSGE KGRDLRFLMN LHNNEAGRTT VFSEMRQECK CHGMSGSCTV
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against WNT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human WNT1

Target Details WNT1

Product Details anti-WNT1 Antibody Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Antigen
Alternative Name WNT1 (WNT1 Antibody Abstract)
Background WNT1 is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT1 is very conserved in evolution, and it is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. WNT1 was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. WNT1 is clustered with another family member, WNT10B, in the chromosome 12q13 region.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: INT1
Protein Interaction Partner: FZD8, LRP6, POLR2C, ROR2, WIF1, SFRP2, SFRP1, PORCN, WNT3A, LRP5, FZD9, RYK,
Protein Size: 370
Molecular Weight 38 kDa
Gene ID 7471
NCBI Accession NM_005430, NP_005421
UniProt P04628
Pathways WNT Signaling, Dopaminergic Neurogenesis

Application Details

Product Details anti-WNT1 Antibody Target Details WNT1 Handling References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 370 AA

Restrictions For Research Use only

Handling

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-WNT1 Antibody (ABIN2779465)

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling Images back to top
Background publications

David, Brenner, Stieber, Schwarz, Brunner, Vollmer, Mentele, Müller-Höcker, Kitajima, Lickert, Rupp, Franz: "MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling." in: Nature cell biology, Vol. 10, Issue 3, pp. 338-45, 2008

Images

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) back to top
Supplier Images
Western Blotting (WB) image for anti-WNT1 antibody (Wingless-Type MMTV Integration Site Family, Member 1) (Middle Region) (ABIN2779465) WB Suggested Anti-WNT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-WNT1 antibody (Wingless-Type MMTV Integration Site Family, Member 1) (Middle Region) (ABIN2779465) Sample Type: 1. Molecular Weight
2. Control (20ug)
3. shRNA1-WNT1 H9 hES...