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|Application / Reactivity||Human||Mouse (Murine)||Cow (Bovine)||Monkey||Rat (Rattus)||Dog (Canine)||Opossum (Didelphimorphia)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||85 Antibodies||38 Antibodies||19 Antibodies||10 Antibodies||7 Antibodies||6 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies||17 Antibodies||8 Antibodies||3 Antibodies||1 Antibodies||2 Antibodies|
|Antigen||Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Monkey, Mouse (Murine), Opossum (Didelphimorphia), Rat (Rattus) Alternatives|
|Conjugate||This WNT1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-WNT1 AntibodyTarget Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN401405) Images
|Specificity||This antibody reacts with Wnt1 protein.|
Species reactivity (expected):Rat, Bovine, Canine, Macaque, Opossum.
Species reactivity (tested):Human, Mouse.
|Immunogen||Synthetic peptide corresponding to an internal region of Human Wnt1 protein|
Target Details WNT1Product Details anti-WNT1 Antibody Application Details Handling References for anti-WNT1 Antibody (ABIN401405) Images back to top
|Alternative Name||WNT1 (WNT1 Antibody Abstract)|
|Background||The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98 % identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.Synonyms: INT-1, INT1, Proto-oncogene protein Wnt-1|
|Pathways||WNT Signaling, Dopaminergic Neurogenesis|
Application DetailsProduct Details anti-WNT1 Antibody Target Details WNT1 Handling References for anti-WNT1 Antibody (ABIN401405) Images back to top
ELISA: 1/25,000. Western Blot: 1/1,500 - 1/6,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WNT1 Antibody Target Details WNT1 Application Details References for anti-WNT1 Antibody (ABIN401405) Images back to top
|Concentration||1.38 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-WNT1 Antibody (ABIN401405)Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling Images back to top
De Ferrari, Moon: "The ups and downs of Wnt signaling in prevalent neurological disorders." in: Oncogene, Vol. 25, Issue 57, pp. 7545-53, 2006
He, Sheng, Stelter, Li, Zhang, Sinha, Luxon, Xie: "Suppressing Wnt signaling by the hedgehog pathway through sFRP-1." in: The Journal of biological chemistry, Vol. 281, Issue 47, pp. 35598-602, 2006
Tell, Yi, Jockovich, Murray, Hackam: "The Wnt signaling pathway has tumor suppressor properties in retinoblastoma." in: Biochemical and biophysical research communications, Vol. 349, Issue 1, pp. 261-9, 2006
Sievers, Fritzsch, Grzegorczyk, Kuhnen, Müller: "Absolute beta-catenin concentrations in Wnt pathway-stimulated and non-stimulated cells." in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Vol. 11, Issue 3, pp. 270-8, 2006
Yook, Li, Ota, Hu, Kim, Kim, Cha, Ryu, Choi, Kim, Fearon, Weiss: "A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells." in: Nature cell biology, Vol. 8, Issue 12, pp. 1398-406, 2006
ImagesProduct Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN401405) back to top