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anti-Zebrafish (Danio rerio) WNT1 antibody for Western Blotting

Recommended WNT1 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
  • BMND16
  • int-1
  • Int-1
  • INT1
  • Int1
  • int1
  • OI15
  • sb:eu647
  • sw
  • swaying
  • wnt-1
  • WNT-1
  • Wnt-1
  • WNT1
  • wnt1-a
  • Xint-1
  • Xwnt1
  • zgc:194464
  • zgc:194478
Epitope
Middle Region
20
17
8
3
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
105
36
13
5
3
3
3
2
2
1
1
1
Host
Rabbit
91
18
Clonality
Polyclonal
Conjugate
This WNT1 antibody is un-conjugated
5
1
1
1
1
1
Application
Western Blotting (WB)
85
51
31
26
17
14
7
5
2
1
1
1
1
Supplier
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Catalog No. ABIN2779465
$ 289.00
Plus shipping costs $45.00

Top referenced anti-WNT1 antibody for Western Blotting

Similar anti-WNT1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Chicken Zebrafish (Danio rerio)
Western Blotting (WB) 85 Antibodies 29 Antibodies 12 Antibodies 5 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies 10 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 20 Antibodies 5 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 17 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
Epitope Middle Region
(20), (17), (8), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(105), (36), (13), (5), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(91), (18)
Clonality
Polyclonal
Conjugate This WNT1 antibody is un-conjugated
(5), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(85), (51), (31), (26), (17), (14), (7), (5), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT1 Antibody

Target Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) Images
Blocking Peptide Blocking peptide for this product available: ABIN986208
Sequence FGREFVDSGE KGRDLRFLMN LHNNEAGRTT VFSEMRQECK CHGMSGSCTV
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against WNT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human WNT1

Target Details WNT1

Product Details anti-WNT1 Antibody Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Antigen
Alternative Name WNT1 (WNT1 Antibody Abstract)
Background WNT1 is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT1 is very conserved in evolution, and it is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. WNT1 was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. WNT1 is clustered with another family member, WNT10B, in the chromosome 12q13 region.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98 % identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Molecular Weight 38 kDa
Gene ID 7471
NCBI Accession NP_005421, NM_005430
UniProt P04628
Pathways WNT Signaling

Application Details

Product Details anti-WNT1 Antibody Target Details WNT1 Handling References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 370 AA

Restrictions For Research Use only

Handling

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details References for anti-WNT1 Antibody (ABIN2779465) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-WNT1 Antibody (ABIN2779465)

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling Images back to top
Background publications

David, Brenner, Stieber, Schwarz, Brunner, Vollmer, Mentele, Müller-Höcker, Kitajima, Lickert, Rupp, Franz: "MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling." in: Nature cell biology, Vol. 10, Issue 3, pp. 338-45, 2008

Images

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling References for anti-WNT1 Antibody (ABIN2779465) back to top
Supplier Images
Western Blotting (WB) image for anti-WNT1 antibody (Wingless-Type MMTV Integration Site Family, Member 1) (Middle Region) (ABIN2779465) WB Suggested Anti-WNT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-WNT1 antibody (Wingless-Type MMTV Integration Site Family, Member 1) (Middle Region) (ABIN2779465) Sample Type: 1. Molecular Weight
2. Control (20ug)
3. shRNA1-WNT1 H9 hES...