You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human WNT2B antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended WNT2B Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antibodies
  • wnt-2b
  • wnt2b
  • WNT2B
  • WNT13
  • Wnt13
  • XWnt-2
  • Xwnt-2b
  • XWNT2
  • xwnt2b
Epitope
AA 341-391
14
12
11
6
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
57
23
21
5
5
2
2
2
2
2
2
1
1
1
1
Host
Rabbit
57
2
Clonality
Polyclonal
Conjugate
This WNT2B antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
31
21
14
10
5
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN675728
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088487647 ABIN675735 IF (p) FITC Rabbit IgG AA 341-391 Log in to see Polyclonal 1
0.00088487647 ABIN920058 IF (p) Alexa Fluor 488 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN920060 IF (p) Alexa Fluor 555 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN920062 IF (p) Alexa Fluor 350 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN920061 IF (p) Alexa Fluor 647 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN675731 IF (p) Cy3 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN675732 IF (p) Cy5 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN675734 IF (p) Cy7 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN675733 IF (p) Cy5.5 Rabbit IgG AA 341-391 Log in to see Polyclonal
0.00088487647 ABIN2807563 IF (p) Alexa Fluor 594 Rabbit IgG AA 341-391 Log in to see Polyclonal

Top referenced anti-WNT2B antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-WNT2B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 1 Antibodies 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 5 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 8 Antibodies 23 Antibodies
Western Blotting (WB) 8 Antibodies 10 Antibodies 30 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antibodies
Epitope AA 341-391
(14), (12), (11), (6), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(57), (23), (21), (5), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(57), (2)
Clonality
Polyclonal
Conjugate This WNT2B antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(31), (21), (14), (10), (5), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-WNT2B Antibody

Target Details WNT2B Application Details Handling References for anti-WNT2B Antibody (ABIN675728) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human WNT2B
Isotype IgG

Target Details WNT2B

Product Details anti-WNT2B Antibody Application Details Handling References for anti-WNT2B Antibody (ABIN675728) Images back to top
Antigen
Alternative Name Wnt2b (WNT2B Antibody Abstract)
Background

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Subcellular location: Secreted


Synonyms: WNT13, Protein Wnt-2b, Protein Wnt-13, WNT2B
Gene ID 7482
UniProt Q93097
Research Area Cancer, Wnt
Pathways WNT Signaling

Application Details

Product Details anti-WNT2B Antibody Target Details WNT2B Handling References for anti-WNT2B Antibody (ABIN675728) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-WNT2B Antibody Target Details WNT2B Application Details References for anti-WNT2B Antibody (ABIN675728) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-WNT2B Antibody (ABIN675728)

Product Details anti-WNT2B Antibody Target Details WNT2B Application Details Handling Images back to top
Product cited in:

Li, Liu, Su, Ren, Zhu, Tian, Qiu: "MicroRNA-324-3p regulates nasopharyngeal carcinoma radioresistance by directly targeting WNT2B." in: European journal of cancer (Oxford, England : 1990), Vol. 49, Issue 11, pp. 2596-607, 2014

Images

Product Details anti-WNT2B Antibody Target Details WNT2B Application Details Handling References for anti-WNT2B Antibody (ABIN675728) back to top
Supplier Images
SDS-PAGE (SDS) image for anti-WNT2B antibody (Wingless-Type MMTV Integration Site Family, Member 2B) (AA 341-391) (ABIN675728) Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti WNT2B AVPR...
Immunohistochemistry (IHC) image for anti-WNT2B antibody (Wingless-Type MMTV Integration Site Family, Member 2B) (AA 341-391) (ABIN675728) Independently Validated Antibody, image provided by Science Direct, badge number 0294...
'Independent Validation' Badge
Lot Number 120522
Method validated Immunohistochemistry
Positive Control Human kidney
Negative Control Human liver
Notes Faint but distinguishable signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antigen: Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term)
  • Catalog number: ABIN675728
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 120522
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human kidney tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human kidney tissue stained with isotype control
  • Secondary only control: Human kidney tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with anti-WNT2 (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 2: Human liver tissue stained with anti-WNT2 (brown) and counterstained with h...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 3: Human kidney tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 341-391) antibody (ABIN675728) Figure 1: Human kidney tissue stained with secondary antibody only (brown) and counte...