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anti-Rat (Rattus) WNT3 antibody for Enzyme Immunoassay

Recommended WNT3 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
  • Ci-Wnt3
  • Int-4
  • INT4
  • int4
  • wnt-3
  • Wnt-3
  • wnt3
  • WNT3
  • wnt3-A
  • wnt3l
  • Xwnt-3
  • Xwnt3
Epitope
Internal Region
28
12
10
10
8
3
2
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
74
13
8
5
1
1
1
1
Host
Goat
35
20
19
Clonality
Polyclonal
Conjugate
This WNT3 antibody is un-conjugated
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
67
36
20
11
11
8
4
1
1
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Catalog No. ABIN374685
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN614938 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal

Top referenced anti-WNT3 antibody for Enzyme Immunoassay

Similar anti-WNT3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 5 Antibodies 8 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 3 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 5 Antibodies
Western Blotting (WB) 8 Antibodies 12 Antibodies
Immunochromatography (IC) 8 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
Epitope Internal Region
(28), (12), (10), (10), (8), (3), (2), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(74), (13), (8), (5), (1), (1), (1), (1)
Host Goat
(35), (20), (19)
Clonality
Polyclonal
Conjugate This WNT3 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(67), (36), (20), (11), (11), (8), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT3 Antibody

Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN374685) Images
Specificity This antibody recognizes WNT3.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. Genename: WNT3

Target Details WNT3

Product Details anti-WNT3 Antibody Application Details Handling References for anti-WNT3 Antibody (ABIN374685) Images back to top
Antigen
Alternative Name WNT3 (WNT3 Antibody Abstract)
Background Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult. It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer). Wnt3 and Wnt3a are nearly 90 % identical at the amino acid level.Synonyms: INT-4, INT4, Proto-oncogene protein Wnt-3
Gene ID 7473, 9606
UniProt P56703
Pathways WNT Signaling

Application Details

Product Details anti-WNT3 Antibody Target Details WNT3 Handling References for anti-WNT3 Antibody (ABIN374685) Images back to top
Application Notes Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details References for anti-WNT3 Antibody (ABIN374685) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-WNT3 Antibody (ABIN374685)

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling Images back to top
Background publications

Okamoto, Inoue, Iwamura, Terashima, Soya, Asashima, Kuwabara: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011

Images

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN374685) back to top
Supplier Images
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN374685) anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody
 image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN374685) anti-Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) (Internal Region) antibody (Image 2)