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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||6 Antibodies||9 Antibodies||37 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies||6 Antibodies|
|Western Blotting (WB)||10 Antibodies||14 Antibodies|
|Immunochromatography (IC)||8 Antibodies|
|Antigen||Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This WNT3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-WNT3 AntibodyTarget Details WNT3 Application Details Handling Images
|Specificity||This antibody recognizes WNT3.|
Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. Genename: WNT3|
Target Details WNT3Product Details anti-WNT3 Antibody Application Details Handling Images back to top
|Alternative Name||WNT3 (WNT3 Antibody Abstract)|
|Background||Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult. It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer). Wnt3 and Wnt3a are nearly 90 % identical at the amino acid level.Synonyms: INT-4, INT4, Proto-oncogene protein Wnt-3|
|Gene ID||7473, 9606|
|Pathways||WNT Signaling, Regulation of Cell Size|
Application DetailsProduct Details anti-WNT3 Antibody Target Details WNT3 Handling Images back to top
Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WNT3 Antibody Target Details WNT3 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|