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|Antigen||Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies|
|Epitope||AA 184-303 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This WNT5A antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-WNT5A AntibodyTarget Details WNT5A Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Protein Wnt-5a(WNT5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Protein Wnt-5a(WNT5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: wingless-type MMTV integration site family, member 5A
Protein Name: Protein Wnt-5a
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Wnt5a recombinant protein (Position: D184-D303). Human Wnt5a shares 100% amino acid (aa) sequences identity with both mouse and rat Wnt5a.|
Target Details WNT5AProduct Details anti-WNT5A Antibody Application Details Handling Images back to top
|Alternative Name||WNT5A (WNT5A Antibody Abstract)|
Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. This gene is a member of the WNT gene family. The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation. It is mapped to 3p14.3. WNT5A is highly expressed in the dermal papilla of depilated skin. This protein plays an essential role in regulating developmental pathways during embryogenesis. And this protein may also play a role in oncogenesis. WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.
Synonyms: hWNT 5A antibody|hWNT5A antibody|Protein Wnt 5a antibody|Protein Wnt-5a antibody|Protein Wnt5a antibody|Wingless type MMTV integration site family member 5A antibody|Wnt 5a antibody|WNT 5A protein antibody|WNT 5A protein precursor antibody|WNT5A antibody|WNT5A protein precursor antibody|WNT5A_HUMAN antibody
|Pathways||WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation|
Application DetailsProduct Details anti-WNT5A Antibody Target Details WNT5A Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for Wnt5a is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-WNT5A Antibody Target Details WNT5A Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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