anti-Eukaryotic Translation Termination Factor 1 (ETF1) Antibodies

ETF1 encodes a class-1 polypeptide chain release factor. Additionally we are shipping Eukaryotic Translation Termination Factor 1 Proteins (17) and Eukaryotic Translation Termination Factor 1 Kits (10) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ETF1 2107 P62495
ETF1 225363 Q8BWY3
ETF1 307503 Q5U2Q7
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Top anti-Eukaryotic Translation Termination Factor 1 Antibodies at antibodies-online.com

Showing 10 out of 36 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB Rabbit Anti-ETF1 Antibody Catalog Number: ARP54738_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in bile ductule Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec WB Suggested Anti-ETF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: THP-1 cell lysate 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: eRF1 Antibody [NBP1-31113] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000. Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details
Human Mouse Un-conjugated CyTOF, ELISA, FACS, ICC, IHC, WB Flow Cytometry: eRF1 Antibody (4F9H12)  - Analysis of Hela cells using RF1 mouse mAb (green) and negative control (red). Western Blot: eRF1 Antibody (4F9H12)  - Analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa) 0.1 mg Log in to see 7 to 9 Days
$516.98
Details
Human Mouse Un-conjugated FACS, WB HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1. 0.1 mL Log in to see 2 to 3 Days
$482.90
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31144] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution. Western Blot: eRF1 Antibody [NBP1-31144] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE eRF1 antibody, antibody diluted at 1:1000. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details
Human Mouse Un-conjugated ELISA, WB ETF1 monoclonal antibody (M02), clone 2H4. Western Blot analysis of ETF1 expression in HepG2 . Detection limit for recombinant GST tagged ETF1 is approximately 1ng/ml as a capture antibody. 100 μg Log in to see 11 to 12 Days
$364.00
Details
Human Rabbit Un-conjugated IHC, WB Western Blot: eRF1 Antibody [NBP1-57565] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: THP - 1 cell lysate Immunohistochemistry: eRF1 Antibody [NBP1-57565] - Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in bile ductule Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec 20 μL Log in to see 8 to 11 Days
$233.06
Details
Human Rabbit Un-conjugated IF, IHC, WB Western blot analysis of extracts of various cell lines, using ETF1 antibody (ABIN1876949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. 100 μL Log in to see 11 to 13 Days
$366.77
Details
Human Rabbit Un-conjugated WB ETF1 antibody used at 1 ug/ml to detect target protein. 50 μg Log in to see 9 to 11 Days
$473.93
Details
Dog Mouse Un-conjugated FACS, WB Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200) Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRF1. 0.1 mL Log in to see 8 to 11 Days
$587.60
Details

Top referenced anti-Eukaryotic Translation Termination Factor 1 Antibodies

  1. Human Polyclonal ETF1 Primary Antibody for ICC, IF - ABIN441149 : Bulygin, Bartuli, Malygin, Graifer, Frolova, Karpova: Chemical footprinting reveals conformational changes of 18S and 28S rRNAs at different steps of translation termination on the human ribosome. in RNA (New York, N.Y.) 2016 (PubMed)
    Show all 2 Pubmed References

More Antibodies against Eukaryotic Translation Termination Factor 1 Interaction Partners

Human Eukaryotic Translation Termination Factor 1 (ETF1) interaction partners

  1. Report molecular dynamics free energy calculations on termination complexes, where relative eRF1 binding free energies to different cognate and near-cognate codons are evaluated. The simulations show a high and uniform discrimination against the near-cognate codons, that differ from the cognate ones by a single nucleotide, and reveal the structural mechanisms behind the precise decoding by eRF1.

  2. The GTS loop forms a switch that is key for the multiple codon recognition capability of eRF1.

  3. New information has been presented on architecture of the eRF1 binding site on mammalian ribosome at various translation termination steps and on conformational rearrangements induced by binding of the release factors.

  4. cryo-electron microscopy (cryo-EM) structures at 3.5-3.8 A resolution of ribosomal complexes containing eRF1 interacting with each of the three stop codons in the A-site

  5. We characterized a region of the eRF1 N-terminal domain, the P1 pocket, that we had previously shown to be involved in termination efficiency. We identified two residues, arginine 65 and lysine 109, as critical for recognition of the three stop codons.

  6. C4 lysyl hydroxylation of eRF1 is required for optimal translational termination

  7. The role of the 41 invariant and conserved N-domain residues in stop codon decoding by human eRF1 was determined.

  8. This work provides mechanistic insight into the coordination between GTP hydrolysis by eRF3 and subsequent peptide release by eRF1.

  9. Authors propose that structural variability in the GTS loop may underline the switching between omnipotency and unipotency of eRF1, implying the direct access of the GTS loop to the stop codon.

  10. The NMR data show that the N-domain of human eRF1 exists in two conformational states.

  11. Molecular modeling of eRF1 in the 80S termination complex showed that eRF1 fragments neighboring guanines and adenines of stop signals are compatible with different N domain conformations of eRF1.

  12. By molecular modeling, the eRF1 molecule can be fitted to the A site proximal to the P-site-bound tRNA and to a stop codon in mRNA via a large conformational change to one of its three domains.

  13. Data show that depleting eRF1 increased the Gag-Pol to Gag ratio in cells infected with replication-competent virus.

  14. bacterial polypeptide release factor RF2 is structurally distinct from eukaryotic eRF1

  15. The invariant uridine of stop codons contacts the conserved NIKSR loop in the ribosome

  16. codon dependence of human eRF1 binding to the mRNA-ribosome complex

  17. the intracellular concentration of the eukaryotic release factor 1 (eRF1) is a critical parameter influencing the efficiency of amino acid incorporation by nonsense suppression

  18. Glu55 and Tyr125 residues in the N domain of eRF1 are important for eRF1's decoding function.

  19. we describe a novel complex that contains the NMD factors SMG-1 and Upf1, and the translation termination release factors eRF1 and eRF3 (SURF).

  20. Results shows eRF1 promotes GTP binding by eRF3.

Mouse (Murine) Eukaryotic Translation Termination Factor 1 (ETF1) interaction partners

  1. Retroviral insertional mutagenesis identifies the del(5q) genes, CXXC5, TIFAB and ETF1, as well as the Wnt pathway, as potential targets in del(5q) myeloid neoplasms

  2. Reverse Transcriptase enhances suppression of termination and that the interaction of RT with eRF1 is required for an appropriate level of translational readthrough.

Eukaryotic Translation Termination Factor 1 (ETF1) Antigen Profile

Protein Summary

This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X.

Gene names and symbols associated with ETF1

  • eukaryotic translation termination factor 1 (ETF1) antibody
  • eukaryotic translation termination factor 1 (Etf1) antibody
  • eukaryotic translation termination factor 1b (etf1b) antibody
  • eukaryotic translation termination factor 1 (etf1) antibody
  • eukaryotic translation termination factor 1 S homeolog (etf1.S) antibody
  • eukaryotic peptide chain release factor subunit 1 (Tb11.22.0012) antibody
  • eukaryotic peptide chain release factor subunit 1 (THAPSDRAFT_22240) antibody
  • eukaryotic peptide chain release factor subunit 1 (TTHERM_00292170) antibody
  • eukaryotic translation termination factor 1 (LOC692937) antibody
  • eukaryotic petide chain release factor subunit 1 (erf1) antibody
  • eukaryotic release factor 1 (eRF1) antibody
  • Eukaryotic peptide chain release factor subunit 1 (erfa-1) antibody
  • AI463371 antibody
  • CG5605 antibody
  • cl1 antibody
  • D5S1995 antibody
  • D6Ertd109e antibody
  • DDBDRAFT_0188023 antibody
  • DDBDRAFT_0191343 antibody
  • DDB_0188023 antibody
  • DDB_0191343 antibody
  • Dm0184 antibody
  • Dmel\\CG5605 antibody
  • ERF antibody
  • eRF1 antibody
  • Eukaryotic release factor 1 antibody
  • group J antibody
  • hm:zeh0057 antibody
  • l(3)00103 antibody
  • l(3)05637 antibody
  • l(3)77ABb antibody
  • l(3)neo28 antibody
  • RF1 antibody
  • RRF1 antibody
  • SUP45L1 antibody
  • TB3-1 antibody
  • wu:fa19e07 antibody
  • wu:fi31f01 antibody
  • zeh0057 antibody

Protein level used designations for ETF1

eukaryotic peptide chain release factor subunit 1 , polypeptide chain release factor 1 , protein Cl1 , sup45 (yeast omnipotent suppressor 45) homolog-like 1 , eukaryotic release factor 1 , etID39694.10 , eRF1 , SUP1 homolog , omnipotent suppressor protein 1 homolog , suppressor , eukaryotic polypeptide chain release factor 1 , eukaryotic translation termination factor 1 , CG5605-PA , CG5605-PB , CG5605-PC , CG5605-PE , CG5605-PF , CG5605-PG , CG5605-PH , CG5605-PI , eRF1-PA , eRF1-PB , eRF1-PC , eRF1-PE , eRF1-PF , eRF1-PG , eRF1-PH , eRF1-PI , Eukaryotic release factor 1 , Protein Cl1

GENE ID SPECIES
2107 Homo sapiens
225363 Mus musculus
266989 Danio rerio
307503 Rattus norvegicus
394632 Xenopus (Silurana) tropicalis
399462 Xenopus laevis
541077 Bos taurus
3664379 Trypanosoma brucei brucei strain 927/4 GUTat10.1
7453361 Thalassiosira pseudonana CCMP1335
7839615 Tetrahymena thermophila
100009553 Oryctolagus cuniculus
416182 Gallus gallus
462098 Pan troglodytes
692937 Bombyx mori
8626719 Dictyostelium discoideum AX4
100020176 Monodelphis domestica
100174012 Pongo abelii
40240 Drosophila melanogaster
101838018 Mesocricetus auratus
100717687 Cavia porcellus
179028 Caenorhabditis elegans
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