anti-Histone Cluster 1, H4a (HIST1H4A) Antibodies

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Additionally we are shipping and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HIST1H4A 364716  
HIST1H4A 8359 P62805
HIST1H4A 326619 P62806
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Top anti-Histone Cluster 1, H4a Antibodies at antibodies-online.com

Showing 10 out of 76 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated WB 400 μL Log in to see 10 to 11 Days
$335.50
Details
Human Rabbit Un-conjugated WB H4-K20 Antibody (N-term) (ABIN651847) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the H4 antibody detected the H4-K20(arrow). From left to right ,the Sample Lot# is SA090513AM or SA090513AN . H4-K20 Antibody (N-term) (ABIN651847) western blot analysis in mouse NIH-3T3 cell line lysates (35 µg/lane). This demonstrates the H4 antibody detected the H4-K20 (arrow). From left to right ,the Sample Lot# is SA090513AM or SA090513AN . 400 μL Log in to see 10 to 11 Days
$335.50
Details
Human Rabbit Un-conjugated DB, FACS, IHC (p) Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab ( ABIN650815) on nitrocellulose membrane.  50 ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL. Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$335.50
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of lysates from A431,mouse NIH/3T3,L929,rat C6,Hela cell line (from left to right), using HIST1H4A Antibody (C-term)(ABIN5969741). ABIN5969741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Flow cytometric analysis of MCF-7 cells using HIST1H4A Antibody (C-term)(green, Cat#ABIN5969741) compared to an isotype control of rabbit IgG(blue). ABIN5969741 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. 80 μL Log in to see 10 to 11 Days
$120.00
Details
Human Mouse Un-conjugated ChIPSeq, ChIP, DB, IF, WB ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the GAPDH promoter and a region located 1 kb upstream of the GAPDH promoter, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). The figure shows the peak distribution in genomic regions from chromosomes 12, 7, 14 and 3 surrounding the GAPDH, ACTB, FOS and EIF4A2 positive control genes. 50 μg Log in to see 11 to 12 Days
$648.60
Details
Human Mouse Un-conjugated ChIP, DB, IF, WB Cross reactivity tests using the Histone H4 (K20me2) antibody.  Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest. Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells. 50 μg Log in to see 11 to 12 Days
$648.60
Details
Human Rabbit Un-conjugated ChIP, DB, ELISA, IF, WB ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H4 (K8ac), crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:16700. Cross reactivity test using the Histone H4 (K8ac) antibody.  Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest. 50 μg Log in to see 11 to 12 Days
$648.60
Details
Human Rabbit Un-conjugated ChIPSeq, ChIP, DB, ELISA, IF, WB ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). ChIP was performed on sheared chromatin from 1 million HeLa cells. The figure shows the signal distribution along the complete length of chromosome 1 and a zoomin to a 500 kb region and the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls. The position of the amplicon used for validating the QPCR results is shown with an arrow. 50 μg Log in to see 11 to 12 Days
$648.60
Details
Human Rabbit Un-conjugated ChIPSeq, ChIP, DB, ELISA, IF ChIP assays were performed using human HeLa cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). ChIP was performed on sheared chromatin from 1 million HeLa cells. The figure shows the signal distribution along the complete length of chromosome 5 and a zoomin to a 600 kb region and the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls. The position of the amplicon used for validating the QPCR results is shown with an arrow. 50 μg Log in to see 11 to 12 Days
$648.60
Details

Top referenced anti-Histone Cluster 1, H4a Antibodies

  1. Human Polyclonal HIST1H4A Primary Antibody for WB - ABIN1944854 : Sierra, Stein, Stein: Structure and in vitro transcription of a human H4 histone gene. in Nucleic acids research 1983 (PubMed)
    Show all 5 Pubmed References

More Antibodies against Histone Cluster 1, H4a Interaction Partners

Human Histone Cluster 1, H4a (HIST1H4A) interaction partners

  1. We found different rejection-related genes between allograft biopsies and peripheral blood. One overlapping gene, HIST1H4A, was consistently expressed in allograft biopsy samples and blood of kidney transplant recipients.

  2. Data indicate that minichromosome maintenance protein 2 (MCM2 (show MCM2 Antibodies)) binding is not required for incorporation of histone H3.1 (show HIST1H3A Antibodies)-H4 into chromatin but is important for stability of H3.1-H4.

  3. hybrid exercise increases expression of eukaryotic translation initiation factor 5A (show EIF5A Antibodies) (EIFSA), peroxisomal biogenesis factor 6 (PEX6 (show PEX6 Antibodies)) and histone cluster 1 H4 (HIST1H4), compared with electrical stimulation alone

Histone Cluster 1, H4a (HIST1H4A) Antigen Profile

Protein Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Gene names and symbols associated with HIST1H4A

  • histone cluster 1, H3f (Hist1h3f) antibody
  • histone cluster 1 H4 family member a (HIST1H4A) antibody
  • histone cluster 1, H4a (Hist1h4a) antibody
  • H4FA antibody
  • Hist1h4a antibody

Protein level used designations for HIST1H4A

histone 1, H4a , histone cluster 1, H4a , H4 histone family, member A , histone H4

GENE ID SPECIES
364716 Rattus norvegicus
8359 Homo sapiens
326619 Mus musculus
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